-
-
Figure 1. Mapping axonal projections in a rhesus macaque brain with VISoR imaging and SMART pipeline
A: Schematic of VISoR microscope performing automated 3D imaging. B: SMART pipeline of serial sectioning and clearing, 3D microscopy, and semi-automated reconstruction and tracing. C: Axonal projections in macaque brain. Scale bar: 5 mm.
-
[1] Chen HZ, Yang HY, Zhong K, Li JL. 2020. Preliminary study on fine structures of subcortical nuclei in rhesus monkeys by ex vivo 9.4 T MRI. Zoological Research, 41(2): 199−202. doi: 10.24272/j.issn.2095-8137.2020.013 [2] Gao L, Liu S, Gou LF, Hu YC, Liu YH, Deng L, et al. 2022. Single-neuron projectome of mouse prefrontal cortex. Nature Neuroscience, 25(4): 515−529. doi: 10.1038/s41593-022-01041-5 [3] Peng HC, Xie P, Liu LJ, Kuang XL, Wang YM, Qu L, et al. 2021. Morphological diversity of single neurons in molecularly defined cell types. Nature, 598(7879): 174−181. doi: 10.1038/s41586-021-03941-1 [4] Poo MM, Du JL, Ip NY, Xiong ZQ, Xu B, Tan T. 2016. China brain project: basic neuroscience, brain diseases, and brain-inspired computing. Neuron, 92(3): 591−596. doi: 10.1016/j.neuron.2016.10.050 [5] Ueda HR, Dodt HU, Osten P, Economo MN, Chandrashekar J, Keller PJ. 2020. Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy. Neuron, 106(3): 369−387. doi: 10.1016/j.neuron.2020.03.004 [6] Van Essen DC. 2020. A 2020 view of tension-based cortical morphogenesis. Proceedings of the National Academy of Sciences of the United States of America, 117(52): 32868−32879. doi: 10.1073/pnas.2016830117 [7] Wang H, Zhu QY, Ding LF, Shen Y, Yang CY, Xu F, et al. 2019. Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution. National Science Review, 6(5): 982−992. doi: 10.1093/nsr/nwz053 [8] Winnubst J, Bas E, Ferreira TA, Wu ZH, Economo MN, Edson P, et al. 2019. Reconstruction of 1, 000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain. Cell, 179(1): 268−281.e13. doi: 10.1016/j.cell.2019.07.042 [9] Xu F, Shen Y, Ding LF, Yang CY, Tan H, Wang H, et al. 2021. High-throughput mapping of a whole rhesus monkey brain at micrometer resolution. Nature Biotechnology, 39(12): 1521−1528. doi: 10.1038/s41587-021-00986-5 [10] Xu R, Bichot NP, Takahashi A, Desimone R. 2022. The cortical connectome of primate lateral prefrontal cortex. Neuron, 110(2): 312−327.e7. doi: 10.1016/j.neuron.2021.10.018 [11] Zhou C, Yang XQ, Wu SH, Zhong QY, Luo T, Li AA, et al. 2022. Continuous subcellular resolution three-dimensional imaging on intact macaque brain. Science Bulletin, 67(1): 85−96. doi: 10.1016/j.scib.2021.08.003 [12] Zhou Y, Sharma J, Ke Q, Landman R, Yuan JL, Chen H, et al. 2019. Atypical behaviour and connectivity in SHANK3-mutant macaques. Nature, 570(7761): 326−331. doi: 10.1038/s41586-019-1278-0 -