印支银乌叶猴(<i>Trachypithecus germaini</i>)的首个参考基因组

Jin-Wei Wu; Ru Zhang; Ya-Lin Qin; Li Wang; Ming-Bo Han; Zi-Fan Zhao; Pei Jing; Hai-Lu Sun; Song Wang; Ning Huang; Bao-Guo Li; Dong-Dong Wu; Chi Zhang; Xiao-Guang Qi

doi:10.24272/j.issn.2095-8137.2022.136

印支银乌叶猴(Trachypithecus germaini)的首个参考基因组

doi: 10.24272/j.issn.2095-8137.2022.136

武进伟^{1, #},
张如^{1, #},
秦雅琳¹,
王力¹,
韩明博¹,
赵子璠¹,
景佩¹,
孙海陆²,
王松³,
黄宁³,
李保国¹,
吴东东⁴,
张弛^5, ,,
齐晓光^1, ,

1.
西北大学生命科学学院, 陕西西安710069, 中国
2.
华大基因, 广东深圳518083, 中国
3.
南宁动物园, 广西南宁 450107, 中国
4.
中国科学院昆明动物研究所, 云南昆明 650223, 中国
5.
中国科学院西北高原生物研究所, 青海西宁810008, 中国

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出版历程
- 收稿日期: 2022-05-31
- 录用日期: 2022-06-14
- 网络出版日期: 2022-06-16
- 刊出日期: 2022-07-18

First reference genome assembly of the Indochinese silvered langur (Trachypithecus germaini)

1.
College of Life Sciences, Northwest University, Xi’an, Shaanxi 710069, China
2.
BGI-Shenzhen, Shenzhen, Guangdong 518083, China
3.
Nanning Zoo, Nanning, Guangxi 450107, China
4.
Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
5.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China

Funds: This work was supported by the National Natural Science Foundation of China (32170512, 31900314, 32001099), Construction Project for Innovation Platform of Qinghai Province (2022-ZJ-Y04), and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31020302)

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Corresponding author: E-mail: qixg@nwu.edu.cn; zhangchi2@bgi.com

#Authors contributed equally to this work

摘要

摘要: 在亚洲叶猴的七个属中，乌叶猴属Trachypithecus是唯一一个包含种组的属。四个种组都各具特点, 石山叶猴种组表现出高钙环境的耐受性，灰叶猴种组具有独特的多夫多妻社会行为，戴帽叶猴种组则具有令人印象深刻的杂交，相比之下，银叶猴种组以其在乌叶猴属分布范围的最南端和银色外观而著称。因此，乌叶猴属Trachypithecus是研究亚洲灵长类进化辐射和行为适应的一个极好模型。然而，由于银叶猴种组参考基因组的长期空缺，难以进行4个种组间的比较基因组学分析。该研究中，基于三代纳米孔Nanopore测序，我们获得了银叶猴种组代表物种印支银乌叶猴(Trachypithecus germaini)从头组装的高质量基因组。该基因组大小为2.91 Gb，Contig 水平的N50达到了55.90 Mb，基因组注释分析共鉴定到了20 332个蛋白质编码基因。印支银乌叶猴T. germaini和石山叶猴种组的代表物种黑叶猴T. francoisi进行基因组比对分析，提示两者的基因组具有良好的共线性关系。通过重建历史有效种群大小，发现印支银乌叶猴的有效种群大小在冰期下降，这可能受到气候变化和人类活动的影响。印支银乌叶猴的高质量基因组，将为进一步深入了解乌叶猴属的自然历史、社会进化以及灵长目动物的适应辐射提供了宝贵的遗传资源。
- 亚洲疣猴 /
- 印支银乌叶猴 (Trachypithecus germaini) /
- 参考基因组
- /
- Trachypithecus germaini /
#Authors contributed equally to this work
注释:

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Figure 1. Geographical distribution and genomic analysis of T. germaini

A: Distribution of T. germaini and Trachypithecus; B: Illustration of T. germaini; C: Genome synteny between T. germaini and T. francoisi; D: Demographic history of T. germaini and T. francoisi. Illustration copyright 2013 Stephen D. Nash/IUCN SSC Primate Specialist Group and Ning Xu, used with permission.

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