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Particulate matter exposure exacerbates susceptibility to SARS-CoV-2 infection in humanized ACE2 mice

Teng-Yu Zhu Huan Qiu Qi-Qi Cao Zi-Lei Duan Feng-Liang Liu Tian-Zhang Song Yang Liu Ya-Qun Fang Guang-Ming Wu Yong-Tang Zheng Wen-Jun Ding Ren Lai Lin Jin

Teng-Yu Zhu, Huan Qiu, Qi-Qi Cao, Zi-Lei Duan, Feng-Liang Liu, Tian-Zhang Song, Yang Liu, Ya-Qun Fang, Guang-Ming Wu, Yong-Tang Zheng, Wen-Jun Ding, Ren Lai, Lin Jin. Particulate matter exposure exacerbates susceptibility to SARS-CoV-2 infection in humanized ACE2 mice. Zoological Research, 2021, 42(3): 335-338. doi: 10.24272/j.issn.2095-8137.2021.088
Citation: Teng-Yu Zhu, Huan Qiu, Qi-Qi Cao, Zi-Lei Duan, Feng-Liang Liu, Tian-Zhang Song, Yang Liu, Ya-Qun Fang, Guang-Ming Wu, Yong-Tang Zheng, Wen-Jun Ding, Ren Lai, Lin Jin. Particulate matter exposure exacerbates susceptibility to SARS-CoV-2 infection in humanized ACE2 mice. Zoological Research, 2021, 42(3): 335-338. doi: 10.24272/j.issn.2095-8137.2021.088

颗粒物暴露加重了人源化ACE2小鼠对SARS-CoV-2感染的易感性

doi: 10.24272/j.issn.2095-8137.2021.088

Particulate matter exposure exacerbates susceptibility to SARS-CoV-2 infection in humanized ACE2 mice

Funds: This work was supported by the National Natural Science Foundation of China (NSFC) (31900331, 32070444), Science and Technology Department of Yunnan Province (202001AW070019), Chinese Academy of Sciences “Light of West China” Program and Youth Innovation Promotion Association (2019378) to L.J. and the NSFC (21761142002), Chinese Academy of Sciences (XDB31000000, SAJC201606 and KGFZD-135-17-011), and Yunnan Province (2019-YT-053 and 2019ZF003) to R.L. and the NSFC (91643206) to W.J.D
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  • 摘要: 由严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2)引起的2019冠状病毒病在全球爆发,全球感染人数超过1.5亿,死亡327万人。有证据表明,可在大气颗粒物(PM)上检测到SARS-CoV-2 的RNA, COVID-19病例与空气污染物水平相关。然而,PM参与SARS-CoV-2传播的机制仍然鲜为人知。我们发现PM暴露增加了一些上皮细胞血管紧张素转换酶2 (angiotensin-converting enzyme 2, ACE2)和跨膜丝氨酸蛋白酶2 (transmembrane serine protease 2, TMPRSS2)的表达水平,并增加了SARS-CoV-2刺突蛋白的吸附。在hACE2小鼠模型中灌注PM显著增加了肺中ACE2Tmpss2的表达和病毒的复制。此外,PM加重了hACE2小鼠中SARS-CoV-2感染引起的肺部病变。综上所述,我们的研究表明,PM是COVID-19的一个流行因素,强调了佩戴防PM口罩应对这场全球大流行的必要性。
    #Authors contributed equally to this work
  • Figure  1.  PM exposure induces ACE2 and TMPRSS2 expression and elevates susceptibility of hACE2 mice to SARS-CoV-2 infection

    A: Immunofluorescence analysis of adsorption spike protein of SARS-CoV-2 after 200 μg/mL PM administration for 24 h. Scale bars: 100 μm. B: Quantitative PCR (qPCR) analysis of ACE2 and TMPRSS2 in HPAEpiC cells after 50 or 200 μg/mL PM administration for 24 h. C: Immunoblot analysis of ACE2 and TMPSSR2 in HPAEpiC cells after 50 or 200 μg/mL PM administration for 24 h. GAPDH was used as the loading control. D: Congestion in lungs of SARS-CoV-2-infected hACE2 mice with or without exposure to PM. Scale bars: 50 μm. E: qPCR analysis of relative viral loads and expression of ACE2 and Tmprss2 in two left pulmonary lobes of mice. F: Immunofluorescence staining of SARS-CoV-2 spike protein and human ACE2 in left lungs of SARS-CoV-2-infected hACE2 mice at 3 dpi. Scale bars: 100 μm. G: qPCR analysis of Il6, Tnfa, Il2, and Il1b genes in SARS-CoV-2-infected hACE2 mice; results are presented relative to those of mouse Hprt. Data are mean±standard error of the mean (SEM).

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出版历程
  • 收稿日期:  2021-03-19
  • 录用日期:  2021-04-09
  • 网络出版日期:  2021-05-08
  • 刊出日期:  2021-05-18

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