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Northern pig-tailed macaques (Macaca leonina) infected with SARS-CoV-2 show rapid viral clearance and persistent immune response

Tian-Zhang Song Hong-Yi Zheng Jian-Bao Han Xiao-Li Feng Feng-Liang Liu Xiang Yang Lin Jin Rong-Hua Luo Ren-Rong Tian Chao Liu Ming-Hua Li Hou-Rong Cai Yong-Tang Zheng

Tian-Zhang Song, Hong-Yi Zheng, Jian-Bao Han, Xiao-Li Feng, Feng-Liang Liu, Xiang Yang, Lin Jin, Rong-Hua Luo, Ren-Rong Tian, Chao Liu, Ming-Hua Li, Hou-Rong Cai, Yong-Tang Zheng. Northern pig-tailed macaques (Macaca leonina) infected with SARS-CoV-2 show rapid viral clearance and persistent immune response. Zoological Research, 2021, 42(3): 350-353. doi: 10.24272/j.issn.2095-8137.2020.334
Citation: Tian-Zhang Song, Hong-Yi Zheng, Jian-Bao Han, Xiao-Li Feng, Feng-Liang Liu, Xiang Yang, Lin Jin, Rong-Hua Luo, Ren-Rong Tian, Chao Liu, Ming-Hua Li, Hou-Rong Cai, Yong-Tang Zheng. Northern pig-tailed macaques (Macaca leonina) infected with SARS-CoV-2 show rapid viral clearance and persistent immune response. Zoological Research, 2021, 42(3): 350-353. doi: 10.24272/j.issn.2095-8137.2020.334

SARS-CoV-2感染北平顶猴(Macaca leonina)呈现病毒快速清除及持续的免疫反应

doi: 10.24272/j.issn.2095-8137.2020.334

Northern pig-tailed macaques (Macaca leonina) infected with SARS-CoV-2 show rapid viral clearance and persistent immune response

Funds: This work was partly supported by the National Key R&D Program of China (2020YFC0842000), National Science and Technology Major Projects of Infectious Disease Funds (2017ZX10304402, 2018ZX10301406-003), the National Natural Science Foundation of China (U1802284, U1902210), National Resource Center for Non-Human Primates
More Information
  • 摘要: SARS-CoV-2感染引起的新型冠状病毒肺炎疫情已成为21世纪全球最重要的公共卫生突发事件。目前,SARS-CoV-2感染非人灵长类动物模型被认为是进行新型冠状病毒肺炎病理损伤特征及致病机制研究的金标准。该研究中,我们成功构建SARS-CoV-2感染北平顶猴(Macaca leonina)实验动物模型,发现北平顶猴可有效支持病毒复制。与恒河猴实验动物模型相比,SARS-CoV-2感染北平顶猴肺组织、鼻拭子、咽拭子及肛拭子中病毒快速清除,这可能与北平顶猴感染后肺组织中干扰素的高表达有关。但是,与恒河猴相比,感染后第二周北平顶猴呈现持续甚至更为严重的炎症反应及临床症状。上述结果表明,病毒清除可能与新型冠状病毒肺炎的疾病进展无关,SARS-CoV-2感染北平顶猴可被视为重症模型用于新型冠状病毒肺炎相关研究。
    #These authors contributed equally to this work
  • 1.  Clearance of SARS-CoV-2 in NPMs

    A: Comparison of viral loads (VL) in nose, rectal, and throat swabs between NPMs and RMs. B: Comparison of viral loads in lung tissues between NPMs and RMs. C: Comparison of IFN-α+, IL-1β+, and IL-6+ cells in lung tissues between NPMs and RMs. Positive ratio=(counts of macaques with SARS-CoV-2 + swabs or lung points/counts of macaques)×100%. *: 0.01<P<0.05; **: 0.001<P≤0.01. ns: No significance. RMs: Rhesus macaques (Macaca mulatta); NPMs: Northern pig-tailed macaques (Macaca leonina).

    Figure  2.  Body injuries and immune response in NPMs

    A: Body temperature in NPMs euthanized at 15 dpi. B: Concentrations of BUN and ALT in serum during SARS-CoV-2 infection (black icon: NPMs euthanized at 7 dpi; gray icon: NPMs euthanized at 15 dpi). C: Number of immune cells in peripheral blood (black icon: NPMs euthanized at 7 dpi; gray icon: NPMs euthanized at 15 dpi). D: Concentrations of cytokines in lung tissue. E: Immunofluorescence staining of immune cells in lungs. *: 0.01<P<0.05; ns: No significance. RMs: Rhesus macaques (Macaca mulatta); NPMs: Northern pig-tailed macaques (Macaca leonina); WBC: White blood cell.

  • [1] Chen L, Liu HG, Liu W, Liu J, Liu K, Shang J, et al. 2020. Analysis of clinical features of 29 patients with 2019 novel coronavirus pneumonia. Chinese Journal of Tuberculosis and Respiratory Diseases, 43(3): 203−208. (in Chinese)
    [2] Gao Y, Li TT, Han MF, Li XY, Wu D, Xu YH, et al. 2020. Diagnostic utility of clinical laboratory data determinations for patients with the severe COVID-19. Journal of Medical Virology, 92(7): 791−796. doi: 10.1002/jmv.25770
    [3] Hadjadj J, Yatim N, Barnabei L, Corneau A, Boussier J, Smith N, et al. 2020. Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients. Science, 369(6504): 718−724. doi: 10.1126/science.abc6027
    [4] Kalaiyarasu S, Kumar M, Kumar DS, Bhatia S, Dash SK, Bhat S, et al. 2016. Highly pathogenic avian influenza H5N1 virus induces cytokine dysregulation with suppressed maturation of chicken monocyte-derived dendritic cells. Microbiology and Immunology, 60(10): 687−693. doi: 10.1111/1348-0421.12443
    [5] Karras A, Thervet E, Legendre C, the Groupe Coopératif de transplantation d'Ile de France. 2004. Hemophagocytic syndrome in renal transplant recipients: report of 17 cases and review of literature. Transplantation, 77(2): 238−243. doi: 10.1097/01.TP.0000107285.86939.37
    [6] Lau SK, Lau CCY, Chan KH, Li CP, Chen HL, Jin DY, et al. 2013. Delayed induction of proinflammatory cytokines and suppression of innate antiviral response by the novel Middle East respiratory syndrome coronavirus: implications for pathogenesis and treatment. The Journal of General Virology, 94(12): 2679−2690. doi: 10.1099/vir.0.055533-0
    [7] Lu SY, Zhao Y, Yu WH, Yang Y, Gao JH, Wang JB, et al. 2020. Comparison of nonhuman primates identified the suitable model for COVID-19. Signal Transduction and Targeted Therapy, 5(1): 157. doi: 10.1038/s41392-020-00269-6
    [8] Mantlo E, Bukreyeva N, Maruyama J, Paessler S, Huang C. 2020. Antiviral activities of type I interferons to SARS-CoV-2 infection. Antiviral Research, 179: 104811. doi: 10.1016/j.antiviral.2020.104811
    [9] Munster VJ, Feldmann F, Williamson BN, van Doremalen N, Pérez-Pérez L, Schulz J, et al. 2020. Respiratory disease in rhesus macaques inoculated with SARS-CoV-2. Nature, 585(7824): 268−272. doi: 10.1038/s41586-020-2324-7
    [10] Ohta H, Yumara-Yagi K, Sakata N, Inoue M, Kawa-Ha K. 1994. Capillary leak syndrome in patients with hemophagocytic lymphohistiocytosis. Acta Paediatrica, 83(10): 1113−1114. doi: 10.1111/j.1651-2227.1994.tb13000.x
    [11] Pang W, Song JH, Lu Y, Zhang XL, Zheng HY, Jiang J, et al. 2018. Host restriction factors APOBEC3G/3F and other interferon-related gene expressions affect early HIV-1 infection in northern pig-tailed macaque (Macaca leonina). Frontiers in Immunology, 9: 1965. doi: 10.3389/fimmu.2018.01965
    [12] Pang W, Zhang GH, Jiang J, Zheng HY, Zhang LT, Zhang XL, et al. 2017. HIV-1 can infect northern pig-tailed macaques (Macaca leonina) and form viral reservoirs in vivo. Science Bulletin, 62(19): 1315−1324. doi: 10.1016/j.scib.2017.09.020
    [13] Schoggins JW, Rice CM. 2011. Interferon-stimulated genes and their antiviral effector functions. Current Opinion in Virology, 1(6): 519−525. doi: 10.1016/j.coviro.2011.10.008
    [14] Shan C, Yao YF, Yang XL, Zhou YW, Gao G, Peng Y, et al. 2020. Infection with novel coronavirus (SARS-CoV-2) causes pneumonia in Rhesus macaques. Cell Research, 30(8): 670−677. doi: 10.1038/s41422-020-0364-z
    [15] Shimizu M. 2019. Clinical features of cytokine storm syndrome. In: Cron RQ, Behrens EM. Cytokine Storm Syndrome. Cham: Springer, 31–41.
    [16] Song TZ, Han JB, Yang X, Li MH, Zheng YT. 2021. Tissue distribution of SARS-CoV-2 in non-human primates after combined intratracheal and intranasal inoculation. Science China Life Sciences. doi: 10.1007/s11427-020-1877-4.
    [17] Song TZ, Zheng HY, Han JB, Jin L, Yang X, Liu FL, et al. 2020. Delayed severe cytokine storm and immune cell infiltration in SARS-CoV-2-infected aged Chinese rhesus macaques. Zoological Research, 41(5): 503−516. doi: 10.24272/j.issn.2095-8137.2020.202
    [18] Woo PCY, Tung ETK, Chan KH, Lau CCY, Lau SKP, Yuen KY. 2010. Cytokine profiles induced by the novel swine-origin influenza A/H1N1 virus: implications for treatment strategies. The Journal of Infectious Diseases, 201(3): 346−353. doi: 10.1086/649785
    [19] Zhang MX, Zheng HY, Jiang J, Song JH, Chen M, Xiao Y, et al. 2018. Northern pig-tailed macaques (Macaca leonina) maintain superior CD4+ T-cell homeostasis during SIVmac239 infection. European Journal of Immunology, 48(2): 384−385. doi: 10.1002/eji.201747284
    [20] Zheng HW, Li H, Guo L, Liang Y, Li J, Wang X, et al. 2020. Virulence and pathogenesis of SARS-CoV-2 infection in rhesus macaques: a nonhuman primate model of COVID-19 progression. PLoS Pathogens, 16(11): e1008949. doi: 10.1371/journal.ppat.1008949
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出版历程
  • 收稿日期:  2020-11-17
  • 录用日期:  2021-04-28
  • 网络出版日期:  2021-05-13
  • 刊出日期:  2021-05-18

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