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A new species of Atelopus (Amphibia: Bufonidae) from eastern Panama

Milan Veselý Abel Batista

Milan Veselý, Abel Batista. A new species of Atelopus (Amphibia: Bufonidae) from eastern Panama. Zoological Research, 2021, 42(3): 272-279. doi: 10.24272/j.issn.2095-8137.2020.319
Citation: Milan Veselý, Abel Batista. A new species of Atelopus (Amphibia: Bufonidae) from eastern Panama. Zoological Research, 2021, 42(3): 272-279. doi: 10.24272/j.issn.2095-8137.2020.319

巴拿马东部发现斑蟾属Atelopus 一新种(两栖纲Amphibia蟾蜍科Bufonidae)

doi: 10.24272/j.issn.2095-8137.2020.319

A new species of Atelopus (Amphibia: Bufonidae) from eastern Panama

Funds: This work was supported by the Sistema Nacional de Investigación (SNI) of SENACYT, Panama, and M.V. received institutional support from Palacky University
More Information
  • 摘要: 本文结合分子、形态和鸣声数据,描述巴拿马东部斑蟾属Atelopus一新种,即A. fronterizo sp. nov. 。新种可依据以下形态特征与同属近缘物种进行区分:1)第一指微弱;2)雌性SVL 35.1–50.1mm(n=13),HW/SVL 0.23–0.34 (n=59), EYND/HW 0.27–0.39 (n=60), TIBL/SVL 0.41–0.56 (n=58), 以及 HAL/SVL 0.22–0.28 (n=49);3)背部底色黄色或绿色,伴有密集的深橄榄色横纹或斑块;4)求偶鸣声长176–235ms,伴有19–34个脉冲,平均脉冲重复率为131.69脉冲/秒,鸣声主频率2422.50–2606.50Hz。新种嵌于中美洲的斑蟾支系中,其与近缘种A. certusA. glyphus在16S和COI线粒体片段上的最小K2P遗传距离均大于2.6%和4.9%。依据16S和COI片段构建的贝叶斯和极大似然法系统演化关系一致,节点支持率较高。依据上述线粒体片段重建的单倍型网络也支持新种自成一支,种内突变步数较低(16S单倍型1-4步,COI单倍型1步),而相比近缘种而言,最低突变步数则普遍较高,其中16S单倍型较A. certus为9步,COI单倍型较A. glyphus为28步。
  • Figure  1.  Molecular comparison and geographic distribution of Panamanian species of Atelopus, and main features of holotype of Atelopus fronterizo sp. nov.

    A: Maximum-likelihood trees based on COI and 16S sequences of Atelopus species from Panamá. Colors of branches indicate bootstrap support, values >90 are marked on branches. B: Map of Panama showing distribution of Atelopus species occurring in the country; C: Atelopus fronterizo sp. nov. holotype (MHCH 3110), dorsal view of preserved specimen; D: Ventral view; E: Head laterally; F: Left hand; G: Left foot; H: Live holotype, from Púcuro River (MHCH 3110); scale bars correspond to 5 mm. All photos were taken by Abel Batista, except for that of A. zeteki, taken by Emanuele Biggi.

    Table  1.   K2P distances for mitochondrial fragments of 16S and COI genes of Atelopus species from Panamá and Colombian samples of A spurrelli.

    Species1234567
    n=2n=2n=3n=1n=7n=19
    1A. fronterizo sp. nov.0.245.18–5.694.93–5.708.88–9.168.64–10.029.74–11.49
    n=40.50–2.04
    2A. certus0.712.41–3.149.48–9.749.98 - 10.029.98–10.57
    n=22.55–3.081.01
    3A. glyphus0.00–0.478.88–9.149.18–9.748.64–9.72
    n=32.55–3.601.52–2.550.00–0.50
    4A. spurrelli0.008.04–8.858.85–11.71
    n=24.67–5.225.755.74–6.290.00
    5A. varius
    n=53.61–4.674.67–5.754.15–5.224.12–4.650.00–0.50
    6A. zeteki0.00–3.900.00–6.50
    n=73.60–5.774.66–6.874.13–6.334.11–5.740.00–2.030.00–2.55
    7A. limosus0.00–6.23
    n=234.15–5.224.67–6.314.15–5.774.12–5.190.50–2.030.50–3.080.00–1.52
    8A. chiriquiensis
    n=14.15–4.694.13–4.673.61–4.154.121.52–2.031.52–3.081.52–2.04
    Ranges represent minimum and maximum observed distances. Intraspecific genetic distances are shown along diagonal of matrix. Values correspond to interspecific genetic distances of 16S and COI genes (COI in bold); COI sequences of A. varius and A. chiriquiensis were not available.
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出版历程
  • 收稿日期:  2020-11-01
  • 录用日期:  2021-03-24
  • 网络出版日期:  2021-03-26
  • 刊出日期:  2021-05-18

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