盘足蝠Eudiscopus denticulus在中国的新发现及其该属系统发育地位的探讨

Wen-Hua Yu; Gabor Csorba; Zheng-Lan-Yi Huang; Yan-Nan Li; Shuo Liu; Rui-Chang Quan; Qiao-Yan Wang; Hong-Yan Shi; Yi Wu; Song Li

doi:10.24272/j.issn.2095-8137.2020.224

盘足蝠Eudiscopus denticulus在中国的新发现及其该属系统发育地位的探讨

doi: 10.24272/j.issn.2095-8137.2020.224

1.
广州大学生命科学学院华南生物多样性保护与利用重点实验室，广东广州 510006，中国
2.
匈牙利自然博物馆动物部，布达佩斯H-1088，匈牙利
3.
中国科学院昆明动物研究所昆明自然历史博物馆，云南昆明 650223，中国
4.
中国科学院西双版纳热带植物园综合保护中心，云南勐腊 666303，中国
5.
西双版纳国家级自然保护区研究中心，云南景洪666100，中国
6.
绵阳师范学院生态安全与保护四川省重点实验室，四川绵阳 621000，中国

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出版历程
- 收稿日期: 2020-08-07
- 录用日期: 2020-12-15
- 网络出版日期: 2020-12-18
- 刊出日期: 2021-01-18

First record of disk-footed bat Eudiscopus denticulus (Chiroptera, Vespertilionidae) from China and resolution of phylogenetic position of the genus

1.
Key Laboratory of Conservation and Application in Biodiversity of South China, School of Life Sciences, Guangzhou University, Guangzhou, Guangdong 510006, China
2.
Department of Zoology, Hungarian Natural History Museum, Budapest H-1088, Hungary
3.
Kunming Natural History Museum of Zoology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
4.
Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, China
5.
Research Institute of Xishuangbanna National Nature Reserve, Jinghong, Yunnan 666100, China
6.
Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Teacher’s College, Mianyang, Sichuan 621000, China

Funds: This study was financially supported by the National Natural Science Foundation of China (31970394, 31670381, 31672258), Guangzhou University’s 2017 Training Program for Young High-Achieving Personnel (BJ201707), Science-Technology Basic Condition Platform from the Ministry of Science and Technology of the People’s Republic of China (2005DKA21402), Lancang-Mekong Cooperation Special Fund (Biodiversity Monitoring and Network Construction Along Lancang-Mekong River Basin Project), and Biodiversity Investigation in Xishuangbanna National Nature Reserve, Biodiversity Investigation, Observation, and Assessment Program (2019-2023) of the Ministry of Ecology and Environment of China (8-2-3-4-5), Scientific Research Foundation of the Education Department of Sichuan Province, China (11ZA164), and Research Foundation of Mianyang Teachers’ College (MYHQ2016A01). Gabor Csorba received support from the National Research, Development, and Innovation Fund of Hungary (NKFIH KH130360) and SYNTHESYS Project, which is financed by the European Community Research Infrastructure Action under the FP7 “Capacities” Program

More Information

Corresponding author: E-mail: wuyizhouq@263.net; lis@mail.kiz.ac.cn

摘要

摘要: 盘足蝠Eudiscopus denticulus (Osgood, 1932)为东南亚地区分布、较稀少的蝙蝠种类。于1981年和2019年夏季，在云南南部进行翼手目调查时，分别采集到8只和3只后足呈盘状的小型蝙蝠。基于外形、头骨形态和分子系统发育学证据证实，这批标本为盘足蝠，为中国翼手目属和种的分布新纪录。以线粒体基因组为标记的系统发育学结果，显示其为鼠耳蝠亚科Myotinae中的基础支系，其基因组编码模式和特征均与鼠耳蝠线粒体基因组类似。同时，课题组也对新采集的个体进行回声定位声波录制与分析。最大熵生态模型MaxEnt预测的结果显示，该物种潜在的适生区呈碎片化，云南南部边境地区均为其潜在的分布区域。
- 盘足蝠Eudiscopus denticulus /
- 中国分布新纪录 /
- 系统发育学位置 /
- 回声定位声波 /
- 适生区预测
Abstract: The disk-footed bat Eudiscopus denticulus (Osgood, 1932) is a rare species in Southeast Asia. During two chiropteran surveys in the summer of 1981 and 2019, eight and three small Myotis-like bats with distinct disk-like hindfeet were collected from Yunnan Province, China, respectively. External, craniodental, and phylogenetic evidence confirmed these specimens as E. denticulus, representing a new genus in China. The complete mitochondrial genome consistently showed robust support for E. denticulus as a basal lineage within Myotinae. The coding patterns and characteristics of its mitochondrial genome were similar to that of other published genomes from Myotis. The echolocation signals of the newly collected individuals were analyzed. The potential distribution range of Eudiscopus in Southeast Asia inferred using the MaxEnt model indicated its potential occurrence along the southern border region of Yunnan, China.
- Eudiscopus denticulus /
- New record from China /
- Resolution of phylogenetic position /
- Echolocation signal /
- Suitable habitat inference

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Figure 1. External (A–C), skull, and dentition (D, E) characteristics of Eudiscopus denticulus from China (GZHU 19159), its potential distribution areas in Southeast Asia predicted by MaxEnt (F), and two maximum-likelihood phylogenetic trees using IQ-tree (G, H)

Live individual (A), ear (B), and hindfoot (C); lateral view of skull and mandible (D); ventral view of skull (E). Scale bar: 5 mm. Photos by Yi Wu (A–C); drawings by Wen-Hua Yu (D, E). F: Black circles mark sampling localities in Xishuangbanna, Yunnan, China; green circles represent historical occurrences from literature and Global Biodiversity Information Facility (GBIF) database (Occurrence dataset https://doi.org/10.15468/igaciv accessed via GBIF.org on 2019-10-14). Red regions are good potential distributions based on localities known so far; orange areas are predictions with inclusion of present records. G: Phylogenetic tree containing 360 presumed vespertilionid species representing large-scale sampling strategy. H: Phylogenetic tree based on 101 complete mitochondrial sequences. Red, green, and blue trapezoids represent Eudiscopus, Submyotodon, and Myotis, respectively.

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Table 1. Descriptive statistics of external and craniodental measurements, and echolocation parameters of Eudiscopus denticulus from China and nearby countries

Index	Yunnan, China	Pu Huong, Vietnam	t-value	Pegu, Myanmar	Laos	PC1	PC2
W (g)	5.2±1.14 (11) (4.0－7.0)	－	－	－	－	－	－
HB (mm)	40.5±2.28 (11) (36.0－45.3)	38.0±2.19 (5) (35.9－40.5)	2.06	－	－	－	－
T (mm)	40.3±2.87 (11) (35.0－45.0)	38.8±2.56 (5) (34.9－41.4)	1.05	－	－	－	－
E (mm)	11.2±1.75 (11) (8.0－13.0)	11.6±0.66 (4) (10.9－12.5)	－0.51	－	－	－	－
HF (mm)	6.0±0.82 (11) (5.4－8.1)	6.7±0.63 (5) (5.8－7.2)	－1.56	－	－	－	－
FA (mm)	36.7±1.10 (11) (34.8－38.5)	34.5±0.88 (5) (33.5－35.6)	3.98*	－	－	－	－
Tib (mm)	17.2±0.54 (11) (16.2－17.9)	16.3±0.64 (5) (15.4－16.9)	2.68*	－	－	－	－
GTL (mm)	14.34±0.34 (8) (13.71－14.79)	14.00±0.22 (10) (13.59－14.32)	2.56*	13.43	14.49	0.81	0.54
CCL (mm)	13.28±0.28 (8) (12.83－13.74)	12.79±0.21 (11) (12.47－13.04)	4.43*	12.15	13.16	0.72	0.62
CBL (mm)	14±0.32 (8) (13.35－14.42)	－	－	－	－	－	－
BCW (mm)	6.84±0.14 (8) (6.63－7.01)	6.74±0.1 (11) (6.53－6.87)	1.76	6.36	6.99	0.46	0.56
BCH (mm)	4.53±0.23 (8) (4.16－4.78)	3.76±0.12 (11) (3.54－3.97)	8.86*	3.07	3.54	0.35	0.72
ZYW (mm)	9.48±0.29 (4) (9.16－9.82)	9.21±0.15 (6) (8.97－9.43)	－	－	－	－	－
MAW (mm)	7.72±0.19 (8) (7.41－7.92)	7.51±0.16 (11) (7.34－7.81)	2.64*	7.20	7.75	0.49	0.75
PL (mm)	6.38±0.11 (8) (6.25－6.6)	－	－	－	－	－	－
IOW (mm)	3.73±0.11 (8) (3.60－3.89)	3.61±0.16 (11) (3.4－3.94)	1.95	3.47	3.71	－0.14	0.87
UIM³L (mm)	6.42±0.18 (8) (6.12－6.68)	－	－	－	－	－	－
UCM³L (mm)	5.44±0.11 (8) (5.31－5.58)	5.36±0.11 (10) (5.21－5.49)	1.61	5.20	5.48	0.88	0.25
UCCW (mm)	3.77±0.06 (8) (3.71－3.91)	3.70±0.09 (9) (3.60－3.91)	1.60^NS	3.59	3.80	0.62	0.53
UM³M³W (mm)	5.86±0.12 (8) (5.62－6.01)	5.81±0.09 (10) (5.74－6.05)	1.24	－	5.88	－	－
LIM₃L (mm)	6.80±0.15 (8) (6.56－7.06)	－	－	－	－	－	－
LCM₃L (mm)	5.74±0.12 (8) (5.54－5.88)	5.70±0.12 (11) (5.52－5.85)	0.77	5.49	5.68	0.89	－0.07
MANL (mm)	10.23±0.18 (8) (9.82－10.42)	10.2±0.15 (11) (9.91－10.46)	0.34	9.74	10.63	0.80	0.30
PCH (mm)	3.29±0.11 (8) (3.13－3.40)	3.16±0.11 (11) (3－3.38)	2.63*	3.00, 3.09	3.25	－	－
HF (kHz)	98.3±3.25 (24) (92.0－104.0)	108.9 (3) (106.5－112.9)^#	－	－	－	－	－
LF (kHz)	49.9±2.02 (24) (45.0－54.4)	52.0 (51.1－53.9)^#	－	－	－	－	－
FMAX (kHz)	53.3±1.29 (24) (50.1－55.0)	61.7 (60.9－63.0)^#	－	－	－	－	－
DUR (ms)	3.1±0.24 (24) (2.8－3.7)	2.03 (1.87－2.11)^#	－	－	－	－	－
Abbreviations can be found in text and Supplementary Materials and Methods. Values are given as means±SD (if n>3) and minimum-maximum (min-max). t-value is from Students t-test between China and Vietnam specimens when measurement distribution fits normality, and * represents P<0.05. Using a Pettersson D500X ultrasound detector (Pettersson Elektronik AB), echolocation calls were recorded from three Chinese-sampled bats (collected in 2019) flying in a room (5 m×4 m×3 m). ^# indicates secondary means and minimum and maximum values based on mean values of three individuals from Zsebők et al. (2014). Note, scores in first parentheses in echolocation measurements indicate number of calls analyzed in this study; those from Zsebők et al. (2014) represent number of individuals in their study. Indices in bold indicate variables used in principal component analysis, PC1 and PC2 scores in bold indicate variables with greatest loadings in respective component. －: Not available.

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