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刘子超, 梁津旸, 兰新强, 李涛, 张家瑞, 赵芳, 李庚, 陈佩仪, 赵云, 李文辉, 赵锋. 2020: 墨江蜈蚣(Scolopendra mojiangica)毒素多样性的比较分析研究. 动物学研究, 41(2): 138-147. DOI: 10.24272/j.issn.2095-8137.2020.019
引用本文: 刘子超, 梁津旸, 兰新强, 李涛, 张家瑞, 赵芳, 李庚, 陈佩仪, 赵云, 李文辉, 赵锋. 2020: 墨江蜈蚣(Scolopendra mojiangica)毒素多样性的比较分析研究. 动物学研究, 41(2): 138-147. DOI: 10.24272/j.issn.2095-8137.2020.019
Zi-Chao Liu, Jin-Yang Liang, Xin-Qiang Lan, Tao Li, Jia-Rui Zhang, Fang Zhao, Geng Li, Pei-Yi Chen, Yun Zhang, Wen-Hui Lee, Feng Zhao. 2020: Comparative analysis of diverse toxins from a new pharmaceutical centipede, Scolopendra mojiangica. Zoological Research, 41(2): 138-147. DOI: 10.24272/j.issn.2095-8137.2020.019
Citation: Zi-Chao Liu, Jin-Yang Liang, Xin-Qiang Lan, Tao Li, Jia-Rui Zhang, Fang Zhao, Geng Li, Pei-Yi Chen, Yun Zhang, Wen-Hui Lee, Feng Zhao. 2020: Comparative analysis of diverse toxins from a new pharmaceutical centipede, Scolopendra mojiangica. Zoological Research, 41(2): 138-147. DOI: 10.24272/j.issn.2095-8137.2020.019

墨江蜈蚣(Scolopendra mojiangica)毒素多样性的比较分析研究

Comparative analysis of diverse toxins from a new pharmaceutical centipede, Scolopendra mojiangica

  • 摘要: 在我国传统中医药记载中,蜈蚣的药用历史有上千年。蜈蚣是一类最古老的有毒动物,其分泌的毒液是重要的捕食、防御的武器,含有大量生物及药理活性分子,极具药用价值。墨江蜈蚣(Scolopendra mojiangica)是一种较为常用的备选药材。本研究通过比较分析,揭示了墨江蜈蚣与药典收录药材—少棘蜈蚣(S. subspinipes mutilans)之间的毒素分子多样性及差异。通过ESI-MS/MS鉴定,并结合毒腺转录组数据分析,在墨江蜈蚣蛋白质组中鉴定了六千多种肽、246种蛋白质分子,其中毒素样蛋白、功能未知的毒素占五分之一。虽然少棘蜈蚣中,毒素蛋白占总蛋白的比例更高,但是通过转录组学比较分析发现,两种蜈蚣毒素转录本的组成非常相似。同时,墨江蜈蚣转录组中鉴定的毒素样蛋白数量是毒液蛋白质组中数量的10倍,由此我们可以推导出更多毒素样多肽的前体结构。墨江蜈蚣毒液的毒性实验发现,其粗毒同样具有很强的溶血活性及杀昆虫活性。我们的研究表明墨江蜈蚣的毒素样蛋白具有丰富的多样性,将为蜈蚣毒素样蛋白的药物开发和利用提供了新的基础。

     

    Abstract: As the oldest venomous animals, centipedes use their venom as a weapon to attack prey and for protection. Centipede venom, which contains many bioactive and pharmacologically active compounds, has been used for centuries in Chinese medicine, as shown by ancient records. Based on comparative analysis, we revealed the diversity of and differences in centipede toxin-like molecules between Scolopendra mojiangica, a substitute pharmaceutical material used in China, and S. subspinipes mutilans. More than 6 000 peptides isolated from the venom were identified by electrospray ionization-tandem mass spectrometry (ESI-MS/MS) and inferred from the transcriptome. As a result, in the proteome of S. mojiangica, 246 unique proteins were identified: one in five were toxin-like proteins or putative toxins with unknown function, accounting for a lower percentage of total proteins than that in S. mutilans. Transcriptome mining identified approximately 10 times more toxin-like proteins, which can characterize the precursor structures of mature toxin-like peptides. However, the constitution and quantity of the toxin transcripts in these two centipedes were similar. In toxicity assays, the crude venom showed strong insecticidal and hemolytic activity. These findings highlight the extensive diversity of toxin-like proteins in S. mojiangica and provide a new foundation for the medical-pharmaceutical use of centipede toxin-like proteins.

     

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