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Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice

Cheng Xiang Zhi-Na Li Tian-Zhuang Huang Jing-Hui Li Lei Yang Jing-Kuan Wei

Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice. Zoological Research, 2019, 40(3): 231-235. doi: 10.24272/j.issn.2095-8137.2019.038
Citation: Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice. Zoological Research, 2019, 40(3): 231-235. doi: 10.24272/j.issn.2095-8137.2019.038

小鼠三个早期发育阶段最大电惊厥阈值(MEST)的异同

doi: 10.24272/j.issn.2095-8137.2019.038

Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice

Funds: This study was supported by the National Natural Science Foundation of China (81403191) and Yunnan Provincial Natural Science Foundation (2018FB118, 2015FA004, and KKSY201626001)
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    Corresponding author: Jing-Kuan Wei
  • 摘要: 人类和其他哺乳动物的大脑,在出生后仍会经历快速的生长发育,并在此过程中逐渐形成完善的大脑功能。本研究利用最大电惊厥阈值检测范式,对产后早期(1-2周龄)、离乳期(3周龄)和青春早期(4-5周龄)的未成年小鼠,开展诱发癫痫大发作行为实验,探索不同发育阶段大脑的神经电生理特点。我们将0.5-4.0 mA、35-200 V范围内不同参数搭配的电刺激给予不同周龄的小鼠后,观察造成其最大电惊厥(MES)、无响应和致死等三种行为学表现的电流、电压范围。结果显示:相较于青春早期小鼠,产后早期小鼠在更宽的电流电压范围(1.0-4.0 mA,60-200V)内均可诱发MES,且无死亡;而离乳期小鼠仅在1.5-2.0 mA,100-160V内发生MES反应,致死范围较其他两组大。此外,产后早期MES反应表现为四肢弯曲,角弓反张,抽搐,与其他两组小鼠躯干和后肢强直,僵硬的行为不一致。总之,本研究通过诱导最大电惊厥阈值实验,发现出生后早期阶段神经系统对电刺激具有更高敏感性和更强耐受性;离乳期时存在关键神经生理调整期。这些结果提示,对于一些儿童多发神经系统疾病的病理和药理机制研究,如儿童癫痫等,应注意区分动物的发育时间,建立更加适用的幼年动物疾病模型。
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出版历程
  • 收稿日期:  2018-08-30
  • 刊出日期:  2019-05-18

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