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Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata

Mei-Er GU Xiao-Ming SONG Chun-Feng ZHU Hong-Ping YIN Gui-Jie LIU Li-Ping YU Wei-Wei YANG Li-Feng NI Yan-Li ZHANG Bao-Jin WU

Mei-Er GU, Xiao-Ming SONG, Chun-Feng ZHU, Hong-Ping YIN, Gui-Jie LIU, Li-Ping YU, Wei-Wei YANG, Li-Feng NI, Yan-Li ZHANG, Bao-Jin WU. Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata. Zoological Research, 2014, 35(4): 249-255. doi: 10.13918/j.issn.2095-8137.2014.4.249
Citation: Mei-Er GU, Xiao-Ming SONG, Chun-Feng ZHU, Hong-Ping YIN, Gui-Jie LIU, Li-Ping YU, Wei-Wei YANG, Li-Feng NI, Yan-Li ZHANG, Bao-Jin WU. Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata. Zoological Research, 2014, 35(4): 249-255. doi: 10.13918/j.issn.2095-8137.2014.4.249

Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata

doi: 10.13918/j.issn.2095-8137.2014.4.249
基金项目: This work was supported by the Public Program of the Science Technology Department, Zhejiang (2011C37077) and the National Natural Science Foundation of China (31071092).
详细信息
    通讯作者:

    Bao-Jin WU

Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata

  • 摘要: In the current study, the alopecia areata gene was introduced into the C57BL/6 (B6) mouse through repeated backcrossing/intercrossing, and the allelic homozygosity of congenic AAtjmice (named B6.KM-AA) was verified using microsatellites. The gross appearance, growth characteristics, pathological changes in skin, and major organs of B6.KM-AA mice were observed. Counts and proportions of CD4+ and CD8+ T lymphocytes in peripheral blood were determined by flow cytometry. Results show that congenic B6.KM-AA mice were obtained after 10 generations of backcrossing/intercrossing. B6.KM-AA mice grew slower than B6 control mice and AA skin lesions were developed by four weeks of age. The number of hair follicles was reduced, but hair structures were normal. Loss of hair during disease progression was associated with CD4+ and CD8+ T lymphocytes infiltration peri-and intra-hair follicles. No pathological changes were found in other organs except for the skin. In the peripheral blood of B6.KM-AA mice, the percentage of CD4+ T cells was lower and percentage of CD8+ T cells higher than in control mice. These findings indicate that B6.KM-AA mice are characterized by a dysfunctional immune system, retarded development and T-cell infiltration mediated hair loss, making them a promising new animal model for human alopecia areata.
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出版历程
  • 收稿日期:  2013-11-25
  • 修回日期:  2013-12-25
  • 刊出日期:  2014-07-08

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