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刘刚, 道日娜, 王蓉蓉, 李云霞, 戴雁峰, 李喜和, 李煜, 李瑶. 2013: 毛脚鵟细胞体外培养体系建立及三种组织来源细胞特性分析. 动物学研究, 34(3): 244-248. DOI: 10.11813/j.issn.0254-5853.2013.3.0244
引用本文: 刘刚, 道日娜, 王蓉蓉, 李云霞, 戴雁峰, 李喜和, 李煜, 李瑶. 2013: 毛脚鵟细胞体外培养体系建立及三种组织来源细胞特性分析. 动物学研究, 34(3): 244-248. DOI: 10.11813/j.issn.0254-5853.2013.3.0244
Gang LIU, Daorina, Rong-Rong WANG, Yun-Xia LI, Yan-Feng DAI, Xi-He LI, Yu LI, Yao LI. 2013: Establishment of cell culture system of rough-legged buzzard and biological characteristic analysis on different tissue cells cultured in vitro. Zoological Research, 34(3): 244-248. DOI: 10.11813/j.issn.0254-5853.2013.3.0244
Citation: Gang LIU, Daorina, Rong-Rong WANG, Yun-Xia LI, Yan-Feng DAI, Xi-He LI, Yu LI, Yao LI. 2013: Establishment of cell culture system of rough-legged buzzard and biological characteristic analysis on different tissue cells cultured in vitro. Zoological Research, 34(3): 244-248. DOI: 10.11813/j.issn.0254-5853.2013.3.0244

毛脚鵟细胞体外培养体系建立及三种组织来源细胞特性分析

Establishment of cell culture system of rough-legged buzzard and biological characteristic analysis on different tissue cells cultured in vitro

  • 摘要: 用组织块培养法对毛脚鵟不同组织进行原代培养,获得了3种不同组织来源的细胞,并成功对细胞进行了冷冻保存和复苏。在传代培养过程中,对比分析了3种组织来源细胞的形态学、生长曲线、贴壁率、核型等生物学特性。形态学方面,3种来源细胞均为成纤维样细胞。对于3种组织来源细胞的贴壁能力分析显示,输卵管源细胞最强,肺源细胞和气管源细胞次之。3种不同组织来源细胞的倍增时间分别为(29.91±0.39)、(33.18±0.21)和(30.67±0.28) h,群体倍增次数分别为 3.54±0.01、4.52±0.02和4.38±0.03。毛脚鵟细胞的染色体数目为2n=68,性染色体为典型的ZW型。本实验为今后毛脚鵟细胞利用、遗传信息的保存及生物学特性的深入研究提供实验材料和依据。

     

    Abstract: In total, three different tissues from the rough-legged buzzard were obtained by culture and successfully cryopreserved and then recovered. During the subculture process, biological characteristics including as cell morphology, growth curve, cell adhesion rate, and karyotype were analyzed and compared, and overall all three kinds of tissue cells exhibited fibroblast-like growth. Oviduct-derived cells had the strongest adherent ability, followed by lung-derived cells and trachea-derived cells. The doubling times of lung-derived cells, trachea-derived cells, and oviduct-derived cells were 29.91?0.39 h, 33.18?0.21 h, and 30.67?0.28 h, respectively, with population doubling times 3.54?0.01, 4.52?0.02, and 4.38?0.03, respectively. Likewise, we noted the chromosome number of the rough-legged buzzard was 68, within the typical type of ZW. These results may potentially provide material and a basis for further research in the field, with the successful preservation of genetic information of rough-legged buzzard.

     

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