Volume 28 Issue 6
Nov.  2007
Turn off MathJax
Article Contents
QIU Chen-li, YANG Gui-bo. Detection of Mamu-DRB*W101 and Mamu-DRB*W201 in Chinese Rhesus Monkeys by PCR-SSP. Zoological Research, 2007, 28(6): 664-669.
Citation: QIU Chen-li, YANG Gui-bo. Detection of Mamu-DRB*W101 and Mamu-DRB*W201 in Chinese Rhesus Monkeys by PCR-SSP. Zoological Research, 2007, 28(6): 664-669.

Detection of Mamu-DRB*W101 and Mamu-DRB*W201 in Chinese Rhesus Monkeys by PCR-SSP

More Information
  • Corresponding author: YANG Gui-bo
  • Received Date: 2007-08-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2007-12-22
  • The aim of this study was to obtain preliminary data about the frequency of 2 MHC class II genes, Mamu-DRB*W101 and Mamu-DRB*W201 among Chinese rhesus macaques. Blood was obtained, and DNA was extracted using QIAamp DNA Blood Mini Kit according to the manufacturer’s instruction. PCR was performed using sequence specific primers to amplify the 2nd exon of Mamu-DRB*W101 and Mamu-DRB*W201. The nucleotide sequences of the amplicons were confirmed by sequencing. In the 136 samples tested in this study, 10 samples were found positive by PCR for Mamu-DRB*W101 and 10 samples positive for Mamu-DRB*W201. The frequency of positive individuals among the Chinese macaques tested was 7.35% for both Mamu-DRB*W101 and Mamu-DRB*W201. The nucleotide sequences of the amplicons were identical to the published sequences. This study demonstrated the existence of both Mamu-DRB*W101 and Mamu-DRB*W201 among Chinese rhesus macaques and provided the basis for large scale typing of these two alleles and studies using Chinese rhesus macaques in SAIDS.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1826) PDF downloads(2037) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return