Volume 29 Issue 1
Jan.  2008
Turn off MathJax
Article Contents
DENG Si-ping, CHEN Song-lin, LIU Ben-wei, XU Jian-yong, TIAN Yong-sheng. Molecular Cloning, Characterization and Expression Analysis of Brain P450arom in Half-smooth Tongue-sole, Cynoglossus semilaevis Gunther. Zoological Research, 2008, 29(1): 17-24.
Citation: DENG Si-ping, CHEN Song-lin, LIU Ben-wei, XU Jian-yong, TIAN Yong-sheng. Molecular Cloning, Characterization and Expression Analysis of Brain P450arom in Half-smooth Tongue-sole, Cynoglossus semilaevis Gunther. Zoological Research, 2008, 29(1): 17-24.

Molecular Cloning, Characterization and Expression Analysis of Brain P450arom in Half-smooth Tongue-sole, Cynoglossus semilaevis Gunther

More Information
  • Author Bio:

    DENG Si-ping

  • Corresponding author: CHEN Song-lin
  • Received Date: 2007-09-16
  • Rev Recd Date: 2007-12-25
  • Publish Date: 2008-02-22
  • The 2184 bp full-length brain P450 aromatase (termed P450aromB) cDNA encoding a protein of 498 amino acid residues was isolated from the brain of the half-smooth tongue-sole (Cynoglossus semilaevis) by using homologous cloning to clarify the role in sex differentiation. Sequence and phylogenetic analyses showed that the tongue sole P450aromB belonged to brain P450arom subfamilies. The putative P450aromB amino acid residues shared 48.3%- 66.1% sequence affinity with other fish brain P450arom, and shared 34.2%-49.9% sequence affinity with ovarian P450arom of other fish. The RT-PCR analysis revealed that P450aromB mRNA was expressed at a high proportion in the brain and gills but less in the gonads and skin, but not in other tissues. However, the P450aromB decreased in the brain of sex-reversed males, treatment by Methyltestosterone or at high temperatures. These results suggested that the P450aromB is involved in the course of gonad development and sex determination in tongue soles.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1867) PDF downloads(1598) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return