Volume 3 Issue 3
May  1982
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SHANG Jin-bao, LIU Wan-yi, QIN Shi-liang, CAI Jue. Studies on the Structure and Function of Amphioxus Ribosomes Ⅰ.Isolation of Amphioxus tRNAs and 5S RNAs. Zoological Research, 1982, 3(3): 317-321.
Citation: SHANG Jin-bao, LIU Wan-yi, QIN Shi-liang, CAI Jue. Studies on the Structure and Function of Amphioxus Ribosomes Ⅰ.Isolation of Amphioxus tRNAs and 5S RNAs. Zoological Research, 1982, 3(3): 317-321.

Studies on the Structure and Function of Amphioxus Ribosomes Ⅰ.Isolation of Amphioxus tRNAs and 5S RNAs

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1982-08-22
  • This article reports the method for preparing Trna and 5S RNA from amphioxus (Branchiostoma belcheri Gray).Whole tissues of frozen amphioxus were homogenized in buffer Ⅰ with a Waring blendor at 0 ℃ for 30s,then the lowmolecular-weight RNAs were extracted by the phenol method.By means of column chromatography of DEAE-celloluse,DEAE-Sephadex A-50 and Sephadex G-100,tRNAs and 5S RNAs were obtained separately.They were further purified by electrophoresis on polyacrylamide gel.The accepting activity of amphioxus tRNA was determined:glycine,6.3%,alanine,5.2% and tyrosine,21%-25%.
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