Mei-Wei ZHAO, Cheng-Li DUAN, Jiang LIU. 2013: Transcription activator-like effectors(TALEs)based genome engineering. Zoological Research, 34(5): 509-518. DOI: 10.11813/j.issn.0254-5853.2013.5.0509
Citation: Mei-Wei ZHAO, Cheng-Li DUAN, Jiang LIU. 2013: Transcription activator-like effectors(TALEs)based genome engineering. Zoological Research, 34(5): 509-518. DOI: 10.11813/j.issn.0254-5853.2013.5.0509

Transcription activator-like effectors(TALEs)based genome engineering

  • Systematic reverse-engineering of functional genome architecture requires precise modifications of gene sequences and transcription levels. The development and application of transcription activator-like effectors(TALEs) has created a wealth of genome engineering possibilities. TALEs are a class of naturally occurring DNA-binding proteins found in the plant pathogen Xanthomonas species. The DNA-binding domain of each TALE typically consists of tandem 34-amino acid repeat modules rearranged according to a simple cipher to target new DNA sequences. Customized TALEs can be used for a wide variety of genome engineering applications, including transcriptional modulation and genome editing. Such "genome engineering" has now been established in human cells and a number of model organisms, thus opening the door to better understanding gene function in model organisms, improving traits in crop plants and treating human genetic disorders.
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