Abstract: The gonadotropin (GTH) secretion from pituitary fragments of helmet catfish (Cranoglanis bouderius) was studied by a perfusion incubation system and GTH radiommunoassay (RIA).The results showed that chronic administration of gonadotropin hormone-releasing hormone analogs (GnRH-A) was able to stimulate GTH release from pituitary fragments in vitro,which was consistent to African catfish (Clarias gariepinus) and catfish (Silurus asotus).The insensitivity to chronic administration of GnRH-A was not be observed in the helmet catfish，which was consistent to catfish and African catfish，but different from that in goldfish (Carassius auratus) and Cyprinus carpio haematopters.Repetitive pulse administration of GnRH-A was also able to stimulate GTH release from the pituitary fragments in dose-dependent manner in helmet catfish.These results showed that chronic administration of GnRH-A could have an effect on artificial propagation of helmet catfish.