Abstract: The total mRNA was prepared and purified from the venom glands of snake Bungarus fasciatus.A cDNA library of the venom glands was then constructed using reverse transcription.Two cDNAs encoding two novel phospholipase A2,named as Bungarus fasciatus phospholipase A2Ⅰ(Bf-PLA2Ⅰ) and Bungarus fasciatus phospholipase A2 Ⅱ(Bf-PLA2Ⅱ) respectively,were screened out by polymerase chain reaction and sequenced.Both cDNAs have an open reading frame of 435 bp,encoding a 145 amino acid phospholipase A2 precursor composed of a 27 amino acid signal peptide followed by 118 amino acid mature protein.The two cloned phospholipase A2 belong to Group Ⅰ phospholipase A2.Their primary structures are different from known Bungarus fasciatus phospholipase A2s.