从细胞色素b基因序列探讨我国林蛙属动物的系统发生关系

Phylogenetic Relationships of Chinese Brown Frogs (Rana) Based on Sequence of Mitochondrial Cytochrome b Gene

摘要: 对中国林蛙属动物进行了DNA水平的分子系统发生研究，测定了中国林蛙属7种15个样品、侧褶蛙属2种2个样品Cytb基因长约360 bp片段的序列。对这些序列的系统发生分析表明：①中国林蛙甘肃种群与东北种群序列差异较大，但尚难根据序列差异来判断它们是不同的亚种还是不同的物种；②7种林蛙中，中国林蛙与黑龙江林蛙的亲缘关系最近，与桓仁林蛙最远；③支持将昭觉林蛙、镇海林蛙、峨眉林蛙和长肢林蛙归入日本林蛙种组Rana japonica group。

Abstract: Fragments of about 360 base pairs of the mitochondrial cytochrome b gene from 7 species of Rana and 2 species of Pelophylax were amplified and sequenced.Phylogenetic analyses reveal that:1)The DNA sequence variations between Gansu population and Northeastern population of Rana chensinensis are notable,but the data are insufficient to judge whether the two populations are two different subspecies or species.2)Rana chensinensis is most closely related to Rana amurensis,and is most alienated from R.huanrenensis among the 7 species of Rana studied.3)The molecular evidences support to place R.zhenhaiensis,R.omeimo ntis,R.chaochiaoensis and R.longicrus into Rana japonica species group.