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张亚平. 1996: 马来熊的DNA序列分析与遗传多样性研究(英文). 动物学研究, 17(4): 459-468.
引用本文: 张亚平. 1996: 马来熊的DNA序列分析与遗传多样性研究(英文). 动物学研究, 17(4): 459-468.
ZHANG Ya-ping. 1996. Genetic Variability and Conservation Relevance of The Sun Bear As Revealed by DNA Sequences. Zoological Research, 17(4): 459-468.
Citation: ZHANG Ya-ping. 1996. Genetic Variability and Conservation Relevance of The Sun Bear As Revealed by DNA Sequences. Zoological Research, 17(4): 459-468.

马来熊的DNA序列分析与遗传多样性研究(英文)

Genetic Variability and Conservation Relevance of The Sun Bear As Revealed by DNA Sequences

  • 摘要: 马来熊在IUCN红皮书中被列为受胁动物,其保护受到广泛的关注。本文研究4只马来熊的线粒体DNA序列,其中1只来自云南,其余3只产地不详,但来自不同的搜集渠道。对于每个个体,我们测定了397 bp的细胞色素b基因、346 bp的12S rRNA基因、98 bp的tRNA基因和333 bp的D环区序列,共计1174 bp。经与黑犀牛序列比较,发现RNA基因的空间结构对基因的进化有显著影响,环区的进化明显快于茎区的。对于细胞色素b基因、12S rRNA基因和tRNA基因,在马来熊个体间未发现序列变异。这一结果提示,马来熊群体的遗传变异程度较低。在D环区,有13和1个位点分别出现转换和颠换。根据D环区序列,我们采用简约法确定了马来熊群体间的进化关系。我们的结果表明,线粒体DNA的D环区是研究马来熊群体遗传结构十分有效的遗传标记。

     

    Abstract: Sun bear is found in Burma,Thailand,Vietnam,Sumatra,Borneo,North-east of India,Malaysia,and Yunnan and Sichuan of China and has been listed in 1990 IUCN Red List of Threatened Animals as a vulnerable species.In this paper,a total of 1174 bases of mitochondrial DNA sequence (397 bases of cytochrome b gene,346 bases of 12S Rrna gene,98 bases of Trna genes,and 333 bases of D-loop region) from each of four sun bears from different collections have been sequenced.Comparing with the black rhino Trna genes,our results suggest that the loop regions of Trna genes evolve much fast than the stem regions.In the cytochrome b gene,12S Rrna gene and Trna gene regions,no sequence variation among the individuals was observed,which indicates that genetic variability in the sun bear is relatively low.In the D-loop region,there are 13 and 1 sites,respectively,characterized by transition and transversion among the 4 sun bears sequenced.The relationships among all the individuals were fully resolved based on mitochondrial D-loop sequences by using parsimony analysis.Our results suggest that the mitochondrial D-loop region is very useful for population genetic study of the sun bear.

     

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