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王寿兵, 张思路, 屈云芳, 经佐琴, 蒋朝光, 吴千红. 1996: 温度变化中国林蛙胚胎发育的影响. 动物学研究, 17(4): 411-419.
引用本文: 王寿兵, 张思路, 屈云芳, 经佐琴, 蒋朝光, 吴千红. 1996: 温度变化中国林蛙胚胎发育的影响. 动物学研究, 17(4): 411-419.
WANG Shou-bing, ZHANG Si-lu, QU Yun-fang, JING Zuo-qing, JIANG Chao-guang, WU Qian-hong. 1996. Effects of Temperature Changce on The Early Embryo Development of Rana chensinensis David. Zoological Research, 17(4): 411-419.
Citation: WANG Shou-bing, ZHANG Si-lu, QU Yun-fang, JING Zuo-qing, JIANG Chao-guang, WU Qian-hong. 1996. Effects of Temperature Changce on The Early Embryo Development of Rana chensinensis David. Zoological Research, 17(4): 411-419.

温度变化中国林蛙胚胎发育的影响

Effects of Temperature Changce on The Early Embryo Development of Rana chensinensis David

  • 摘要: 开展中国林蛙南移和人工养殖是一项很有意义的工作,而南方多变的气温是否影响其胚胎发育是一个尚未研究的课题。本文利用受精卵在不同温度的恒温培养箱间移换,研究了温度变化对中国林蛙早期胚胎发育的影响,结果表明:(1)快速升温对中国林蛙胚胎卵化率、正常率均有影响,且温差越大影响越大;缓慢升温对胚胎的影响与温差大小和升温起点有关,高起点的升温对胚胎的影响比低起点的升温更大,生产中快速升温以不超过2℃,慢速升温以不超过4℃为宜。(2)慢速降温幅度为4℃时,对胚胎发育无影响,为8℃时,有影响;快速降温幅度为4℃时,对胚胎孵化率和正常率均有影响,生产中慢速降温以不超过8℃,快速降温以不超过4℃为宜。(3)变温幅度相同时,变温速度越大,对胚胎的影响越大。(4)原肠期是中国林蛙胚胎发育的升温敏感期,神经管期为升温非敏感期,中国林蛙胚胎发育中对升温变化较降温敏感。(5)中国林蛙的胚胎发育不会因南方天气的异常而遭到严重的影响。

     

    Abstract: It is very promising to develop Rana chensinensis David breeding in South China.Whether the changeable air temperature of the South has an effect on its embryo development is uncertain.We studied the effects of temperature change on early embryo development by moving fertilized eggs between incubators of different temperatures.The results showed:(1) Rapid temperature increase had a harmful influence on hatching and normal rates of the embryos,and the wider the temperature span was,the more harmful the impact was.Effects of moderate temperature increase on embryos were hinged upon the temperature span and starting point.High starting temperature was more harmful.In commercial production,rapid temperature increase should be within 2℃,moderate increase should be within 4℃.(2) In moderate temperature decrease,a span of 4℃ had no influence,but an 8℃ decrease was detrimental.If rapid temperature decrease span was 4℃,both hatching and normal rates were impaired.In commercial production,moderate temperature decrease should be within 8℃ and rapid decrease should be within 4℃.(3) When the change spans were the same,the quicker the change was,the more harmful the impact was.(4) The Gastrula Stage was the time when embryos were sensitive to temperature increase,and the Neural Canal Stage was not.Embryos were more sensitive to temperature increase than to decrease.(5) No serious harm would be imposed upon Rana chensinensis David embryos in development by the changeable climate of South China.

     

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