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马寿祥, 钟金颜. 1982: 紫外光对大白鼠红细胞膜 γ—射线损伤的恢复效应. 动物学研究, 3(1): 95-100.
引用本文: 马寿祥, 钟金颜. 1982: 紫外光对大白鼠红细胞膜 γ—射线损伤的恢复效应. 动物学研究, 3(1): 95-100.
Mashouxiang, zhongjinyan. 1982. Recovery effect of ultraviolet light on the cell menbrane damage of rat erythrocytes induced by gamma-radiation. Zoological Research, 3(1): 95-100.
Citation: Mashouxiang, zhongjinyan. 1982. Recovery effect of ultraviolet light on the cell menbrane damage of rat erythrocytes induced by gamma-radiation. Zoological Research, 3(1): 95-100.

紫外光对大白鼠红细胞膜 γ—射线损伤的恢复效应

Recovery effect of ultraviolet light on the cell menbrane damage of rat erythrocytes induced by gamma-radiation

  • 摘要: 本文研究了紫外、光对细胞膜γ—射线损伤的恢复作用。结果发现经γ—射线300Gy照射后再经紫外光照射5分钟的大白鼠红细胞悬浮液,不但不会加强细胞膜的损伤,反而对细胞膜有明显的恢复作用。经统计处理有显著差别(P<0.05).实验还进一步证明:红细胞悬浮液经γ—射线或紫外光照射后, 在低温中释放的血红蛋白量与照射剂量成正比关系.可作为辐射(包括电离辐射或非电离辐射)对细胞膜损伤的一个方便易行的检查指标.

     

    Abstract: Recovery effect of ultraviolet light on the cell menbrane damage of rat erythrocytes in vitro was studied.Blood samples taken from the rat was centrifuged to separate blood cells from the plasma. The cells were washed twice with isotonic saline, resuspended in normal saline and irradiated. The cell suspensions were exposed to ultraviolet light 5 min 10-20min after γ—irradiation with 60Co γ—ray then kept at 5 ℃ for 20 hours and centrifuged. The hemoglobin of the supernatants was determined. The main results obtained may be summarized as follows:Ultraviolet light decreases radiation-induced hemolysis. The cell suspension of rat erythrocytes exposed to ultraviolet light 5 min 10-20 after irradiation with 300Gy, the radiation-induced hemolysis decreased twice more than that of the control. Hewever, the differences between control and ultraviolet light treated ones are statistically significant (P<0.05).

     

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