Abstract: Mitochondrial gene fragments of 12S and 16S rRNA of six species including 10 populations (Amolops),and 4 outgroup species were sequenced. Aligned sequences showed that there were 940 bp in length with 352 variable sites and 186 parsimony sites. Phylogenetic relationships were analyzed through NJ,MP,and ML methods and got a consistent topological structure,and it indicated that all of the ingroup were clustered together and comprised two groups. In the group Ⅰ, two populations of A.mantzorum were first clustered together as a sister clade to A.loloensis. In the group Ⅱ,A.daiyunensis and A.hongkongensis were clustered together,the Amolops sp. From Lantau Is. Of Hong Kong was clustered with A.ricketii together as a sister group to A.wuyiensis. This result indicates that there are at least two species of Amolops in Hong Kong,and that A.daiyunensis is a valid species.The fact that genetic differentiation between the Hongya and Shimian populations of A.mantzorum is even bigger than that between them and A.loloensis strongly implies that there maybe are some unknown species contained in A.mantzorum.