丙线照射所致人血淋巴细胞与中国仓鼠卵巢成纤维细胞DNA单链断裂及其修复的观察

The Observation of γ-RAY Induced DNA Single Strand Ereaks and Their Rejoining in Chinese Hamster Ovary Cells and Human Peripheral Lymphocytes

摘要: 用微孔滤膜碱洗脱法观察了丙线照射引起人血淋巴细胞(Do为400拉德)及中国仓鼠卵巢成纤维细胞(Do为200拉德)的DNA单链断裂及其修复。在0—3000拉德范围内，两种细胞DNA单链断裂的程度基本一致，照射剂量与单链断裂的对数之间呈直线相关。800、1500及3000拉德照射后，经过0.5—7小时的孵育，中国仓鼠卵巢成纤维细胞DNA单链断裂的修复优于人血淋巴细胞，说明这两种细胞辐射敏感性DNA单链断裂修复能力无关。

Abstract: The induction and rejoining kinetics of DNA single strand breaks in irradiated human peripheral lymphocytes (Do=400 rads) and Chinese hamster ovary cells (CHO)(DO=200 rads)were studied by alkaline elution method.Although the radiosensitivity of these two types of mammalian cells is different,the extent of single strand breakage is approximately the same within the range of 100-3000 rads.The ability to rejoin the single strand breaks in the more radiosensitive CHO cells is greater than that in lymphocytes.It appears that there is no direct relationship between the efficiency of rejoining of sihgle strand break and radiosensitivity in these two types of cells.