过热处理对人类食管癌细胞及中国仓鼠细胞DNA修补能力的抑制作用

Inhibition of DNA Repair Ability of Human Esophageal Cancer Cell and CHO-K1 Cell by Hyperthermia

摘要: 人类食管癌细胞Eca-109在受8，000 rad γ射线照射前，或照射后，用44 ℃处理30分钟，会全部抑制掉随后在37 ℃保温1.5小时内的DNA断链重接修补。照前42℃处理也能起部分的抑制作用；照后42 ℃处理的抑制程度与照前处理的相同，但似乎在42 ℃处理的时间内已完成了不受抑部份的修补。中国仓鼠细胞CHO-K1在紫外线照前受44℃处理30分钟，同样会全部地抑制掉照后37℃保温3小时内的DNA切除修补；照前用42℃处理，也有部份抑制作用。

Abstract: Suspension samples of human esophageal cancer cell strain Eca-109 Were treated,before or after 8,000 rad 60 Co γ-irradiation,with 44° or 42℃ for 30 minutes and then incubated in growth medium at 37℃ for 1.5 hours.The 44° treatment inhibited the DNA rejoining repair completely.The 42℃ treatment inhibited it by about 60%,and the uninhibited portion could be repaired during the 42°treatment after irradiation.The suspension samples of CHO-K1 cell were treated,before UV-irradiation (about 50 J/M2),with 44°or 42℃ for 30 minutes and then incubated in growth medium containing 3H-TdR (1μCi/ml) at 37℃ for 3 hours.The 44°treatment inhibited the DNA excision repair completely.The 42°treatment inhibited it incompletely.