Abstract: In November 1981,we collected a fresh-water dinoflagellate-Glenodinium gymnodinium penard from a pool in the suburbs of Kunming.We have succeeded in the culture of this organism in the laboratory.The medium used is a modified artificial medium for growing Volvox globartor,devised by Provasoli and Pintner (1959).The process of culture is divided into three steps:(1) preliminary culture;(2) pure culture and (3) expand culture.The work is carried out under 8-12 hours illumination each day (the intensity of illumination is at 800-1200 Lux) and at temperature 9-26℃ in the common laboratory.So far,the culture of these cells has been carried to more than 300th progeny.After the culture,we also observed the reproduction form,the growth curve and nuclei of this dinoflagellate,the result showed this organism is a rare dinoflagellate both with a mesocaryotic and an eucaryotic nucleus.