Abstract: The DNAs were extracted from both fresh sample and fur specimens of the Indian muntjac (M.muntjak),the Chinese muntjac (M.reevesi),the black muntjac (M.crinifrons),Feas muntjac (M.feae),and the Gongshan muntjac (M.gongshanensis).For the fur specimens,DNAs of over 1.0 kilobase pair (kb) were obtained.Polymerase Chain Reactions (PCR) were performed for these DNAs using a pair of universal primers localized in cytochrome b gene of animal mitochondrial DNA.Fragments of about 360 base pair (bp) in size were detected by 1.5% agarose gel electrophoresis.We used 28 restriction endonucleases to digest the amplified fragments and found some polymorphic sites among mtDNA of muntjacs.