Abstract: The analysis of calling songs of three frog species from Hangzhou area had been made with computer analysis technique in this paper.The song of Rana nigromaculata was composed of four pulses,the pulse rate (PR) was 8-11 times/sec,the duration of each song was about 370-510 ms,each pulse had three power peaks,and the frequency of highest power peak was between 2.0 and 2.4 kHz.In Rana limnocharis,the PR of the song,composed of 6-7 spindle shape pulses,was 71.7 times/sec,each song had 84.25±7.12 ms duration and its MPF was 1.395±0.023 kHz in each pluse.There were some differences on the structrue,power spectrum and temporal parameters of song between Hangzhous and Shensi Islands.According to the analysis of this experiment,the variant songs of Rana limnocharis had large proportion of pure tones (about 1.28 kHz).The song of Microhyla ornata was composed of 11-13 pulses,and the duration was 16.06±2.08 ms in each pulse.The PR was 36.23 times/sec,the songs duration was 308.64±22.93 ms,the MPF was 1.36±0.02 kHz.As compared with the song characteristics of this frog in Xuancheng area of Anhuei Province,the song of Microhyla ornata in Hangzhou was much different from in Xuancheng area.The difference in physical structure of songs in three species of frogs might be corelated to the structure of their vocal sac,and the reason that the same species in different areas had different song probably was difference of their habitats.