Abstract: This paper reported LDH isozymes about four kinds of tissues of kidney,muscle,liver,and heart in five species of Colubridae:Opheodrys major (Guenther),Natrix tigrina lateralis (Berthold),Zaocys dhumnades (Cantor),Natrix annularis (Hallowell),Dinodon refozonatum (Cantor) by using polyacrylamide gel electrophoresis and optical density scanning.The results indicated that LDH isozyme of snake had tissue specificity and different LDH zymograms in each tissue of the animals,LDH zymograms may be used to identified species as one kind of index of biochemistry.In this paper,using A/B ratio of LDH isozymes of various tissue in five species established the matrix of euclidean distance coefficient and carried it analysis of fuzzy cluster.The reporter discussed preliminary the relation among five species in this paper.