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吴小秋, 张亚平, 丁波, 陈永久, 熊绿芸 何照范, . 1999: 中国主要地方品种猪血液蛋白遗传多样性研究. 动物学研究, 20(4): 252-257.
引用本文: 吴小秋, 张亚平, 丁波, 陈永久, 熊绿芸 何照范, . 1999: 中国主要地方品种猪血液蛋白遗传多样性研究. 动物学研究, 20(4): 252-257.
WU Xiao-qiu, ZHANG Ya-ping, DING Bo, CHEN Yong-jiu, XIONG Lu-yun, HE Zhao-fan. 1999. Genetic Diversity of Blood Protein Among Main Indigenous Pig Breeds in China. Zoological Research, 20(4): 252-257.
Citation: WU Xiao-qiu, ZHANG Ya-ping, DING Bo, CHEN Yong-jiu, XIONG Lu-yun, HE Zhao-fan. 1999. Genetic Diversity of Blood Protein Among Main Indigenous Pig Breeds in China. Zoological Research, 20(4): 252-257.

中国主要地方品种猪血液蛋白遗传多样性研究

Genetic Diversity of Blood Protein Among Main Indigenous Pig Breeds in China

  • 摘要: 实验采用水平板淀粉凝胶电泳技术,对中国地方猪五大类型9个品种、1个引入品种共201个个体进行了遗传多样性分析。共分析遗传座位30个,其中只有MDH、PEPB、AK、6PGD、ADA、PHI 6个座位具有多态性。中国主要地方猪品种的多态座位百分比P=0.200,平均杂合度H=0.065,平均等位基因数A=1.300。根据基因频率采用PHYLIP3.5c软件包计算Nei氏遗传距离,然后用“NEIGHBOR”程序分别构建Neighbor joining(NJ)和UPGMA(un weight pairgroup method with arithmetic mean)系统树。结果表明,中国地方猪品种地理关系远近与遗传关系远近并不完全一致。中国主要地方猪种遗传多样性程度较低。

     

    Abstract: A total of 201 blood samples including 9 indigenous pig breeds (Sus scrofa domesticus) in China and Yorkshine pigs were collected in order to evaluate the genetic diversity among Chinese pig breeds.Thirty blood protein loci were studied by horizontal starch gel electrophoresis,among which 6 loci (MDH,PEPB,AK,6PGD,ADA,PHI) were found to be polymorphic.The percentage of polymorphic loci (P),the mean individual heterozygosity (H) and the mean number of alleles (A) are 0.200,0.065 and 1.300,respectively.The degree of genetic variability of Chinese indigenous pig breeds as a whole was higher than that of goats,lower than those of cattle,horses and sheep.Using gene frequencies of the 30 loci,the pairwise genetic distance among the 9 indigenous breeds in China were calculated by software PHYLIP3.5 c.The phylogenetic trees were constructed using NJ and UPGMA methods.The results suggest that the geographical distribution do not consist with the genetic relationship among Chinese indigenous pig breeds.The genetic diversity among Chinese indigenous pig breeds is low.

     

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