Abstract: In this paper,the coplanar interaction between every two bases in triplet was studied by the empirical interaction potential of multiatomic molecules.We calculated the total energy of base pairs in Watson-Crick hydrogen bond,in Hoogsteen hydrogen bond and in other kind hydrogen bonds (include mismatch base pairs) according to the theoretical model of 22 triplets structures.The conclusion from the analysis of our calculation results was in accordance with the conclusion from experiment results reported.This studies will benefit the further investigation in biological function of multiple hydrogen bond interactions from the theoretical point of view.