Abstract: Using human SRY gene primers,the gene fragment of cattle homologous to human SRY was amplified by PCR.In male cattle,a gene fragment of about 609 bp homologous to human SRY was identified.By ligating pUC-Eco-T with the PCR products and colonies hybridization with human SRY probe,the positive colony with cattle the fragment was identified,and termed pBosY0.6.The colony contains a about 609 bp insert corresponding to human SRY conserved ORF.The restriction map of the recombinant was analysed and compared with that of human SRY gene.