Abstract: The relation between evolution of spatial working memory function and of morphology of the dorsolateral prefrontal cortex among the rhesus monkey (Macaca mulatta),the slow loris (Nycticebus coucang) and the tree shrew (Tupaia belangeri chinensis) were reported in present paper.The results read as follows:In the DR performance with training,the rhesus monkeys and slow lorises could reach a criterion of 90% correct response at 7.7±3.2 seconds,and 3.8±0.4 seconds delay interval,respectively,by 1000 training trails.The tree shrews failed to reach the criterion of 90% correct response even at 0 seconds delay interval by 1000 training trails.If a delay interval was tested in one session (30 trails) only,doing the DR performamce without training,the rhesus monkeys reached a correct of 80% or higher in each session at 0,1,2,3,4,and 5 seconds delya,respectively.The percent correct in each session of the slow lorises showed no differences from the rhesus monkeys at 0,1,2,3,and 4 seconds delay.However,when the delay interval was increased to 5 seconds,the percent correct of the DR performance declined to 70% or lower in the slow lorises.In the tree shrews the percent correct in each session reached to 70% or lower at 0,1,2,3,4,and 5 seconds delay interval,respectively.The morphological studies revealed that the size of the prefrontal cortex increased,and the structure got complex in the course of the evolution in primates.It is suggested that the relation of evolution between the spatial working memory function and anatomy in the prefrontal cortex might be significant among the three species,both the development of morphology and that of the spatial working memory function in the dorsolateral prefrontal cortex are later than other regions of cerebral cortex in phylogenetic evolution course.