Abstract: This report described the fine structure of spermatozoa of Tibetan macaque,a species endemic to China.In the study,transmission electron microscope (TEM),scanning electron microscope (SEM) and light microscope (LM) were employed.The sperm total length was 79.96±0.29 μm,the head of the sperm oval was flat and 5.97±0.05 μm long.The acrosome was large and surround the anterior three-fourths of the nuclear surface.There was a short neck with a centriole and a 11.98±0.05 μm long midpiece consisting of approximately 44 gyres of mitochonndria arranged in an helix.The main piece of the sperm is 62.01±0.32 μm long.The size of the sperm is larger than those of any other species in Macaca (P<0.001).