蟾蜍胰腺内分泌细胞的形态学和体视学研究

Morphological and Stereological Studies of The Langerhans Islet Cells in The Toad pancreas

摘要: 本实验应用免疫组织化学PAP法和免疫金银-PAP法免疫双重标记以及常规电镜，对蟾蜍胰岛A、B、D细胞的形态结构及分布进行了观察；并利用A蛋白胶体金（PAP）免疫电镜技术，对胰岛素进行细胞内精确的定位。同时，根据体视学有关立体重建的原理，从A、B细胞不同大小的切面的频数分布，重建了其球体大小的频数分布。

Abstract: The distribution and morphology of the A,B and D cells in toad pancreas were observed with immunohischemistry (PAP method,PAP-protein A goldsilver double staining) and electron microscopy.By PA-gold (PAG) method,the precise intracellular localization of insulin has been observed.The stereological algorthms for the conversion of the profile-size frequency distribution observed in histological section to the sphere-size frequency distribution in three-dimension space have been developed to a computer program and were applied to A,B cells in the pancreas.