MNNG转化赤麂细胞株(KIZ-8401)的生长特性和核型观察
Observations on New Cell line of Indian muntjac Transformed With Mnng
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摘要: 本文用MNNG (G-Methy-N-nitro-N-nitrosoguanidine)(甲基硝基亚硝基胍)转化赤麂肺成纤维细胞株(KIZ-7901)。转化的赤鹿细胞株(KIZ-8401)具有新的核型,2n=8♂,细胞形态,细胞周期以及生物学特性都与未转化的细胞株(2n=7♂)不一样。转化细胞中还常见多倍体出现,细胞具有克隆生长能力。Abstract: Lung fibroblast line of Indian muntjac (KIZ-7901) was transformed with MNNG (N-Methyl-N-nitro-N-nitrosoguanidine).The transformed Indian muntjac cells (KIZ-8401) possessed the new karyotype with chromosome number of 8 in male and polyploid.The morphology and cell cycle as well as mitotic index in MNNG-transformed muntjac cells were different from diploid cell line of Indian muntjac (KIZ-7901).Moreover,the MNNG-transformed muntjac cells possessed colony-forming capacity in vitro.