Abstract: The rapid identification of shake venom in snakebite is very helpful to the treatment of the snakebite.We have purified three kinds of antibody Fab of snake venoms by affinity chromatography on different immobilized snake venom,in order to avoid crossimmunoreaction.Horseradish peroxidase was coupled to these Fab fragments by the periodate method.We have developed a sandwich enzyme-immunoassay,which is capable of detecting <5 ng of snake venom in about 90 minutes.This assay used in clinical identification of snake venom has achieved satisfied results.