Abstract: The spider crude venom (SV) was extracted from lyophilized poison glands of Lycosa singoriensis and was fractionated by means of chromatography on Sephadex G-25 into 8 fractions.It was shown that the minimal dosa of SV to kill 50% (MD50) of the cultured human lung adenocarcinoma cells (SPC-A1) was 25 μg/ml,whereas even 600 μg/ml of SV killed only 30% of the cultured human embryonic lung cells.Human lymphocytes were nearly as resistant as the embryonic lung cells and MD50 of SV on human erythrocytes was more than 4000 μg/ml.The cytopathic activity on the adenocarcinoma cells was found in fractions Ⅲ,Ⅳ and Ⅷ.Moreover,the cytopathic activity of fraction Ⅳ and Ⅷ was found to be heat resistant (100 ℃,30 min),but that of fraction Ⅲ was disappeared after the heat treatment.