Abstract: This paper reported the treatment of S180 EAC ascites of mice with crotaline snake venoms and antivenin.After inoculating S180,EAC ascites to mice,then injected Agkistrodon acutus and A.halys venoms and Agkistrodon acutus,A.halys antivenin,the injected mice survived longer than control group and their inoculation rates decreased,but they cannot completely prohibit the growth of S180 and EAC ascites.The cancer cells showed the changes of necrosis,fibrosis etc.The analysis of S180 ascites showed the activities of arginine esterase and fibrinolysis.The activities of arginine esterase and fibrinolysis are the same as that of snake crude venoms.The treatment effect of injecting antivenin is the same as that of injecting crude venom,therefore we consider that the crude venoms can treat S180 and EAC ascites may be relative to the antibodies produced by mice.