灵长类淋巴细胞亚群的研究Ⅰ.外周血和淋巴组织的SRBC、SMRBC和ZYC玫瑰花结形成细胞的测定

Studies on Lymphocyte Subpoulations in Primates Ⅰ.The Determination of SRBC,SMRBC and ZYC Rosette Formation Cell in Peripheral Blood and Lymphoid Tissues

摘要: 用经过氨乙基异硫脲(AET)或神经氨酸酶(VCN)处理过的绵羊红细胞(SRBC)，酵母多糖—补体复合物(ZYC)和红面猴红细胞(SMRBC)作为标记，测定了健康恒河猴、熊猴、豚尾猴、红面猴和树lu的外周血以及恒河猴淋巴样组织的淋巴细胞亚群，在这五种动物中，EAET玫瑰花结形成细胞的百分比依次为78.1±6.4,77.7±4.2,76.0±6.4,82和24.9±7.2。ZYC形成细胞的百分比依次为11.4±4.2,9.0±3.8,12.8±2.2,15和15.7±7.4。恒河猴胸腺，脾脏和淋巴结的EAET形成细胞的百分比分别为95.2±2.9,28.8±10.3,44.8±6.2。与人类不同，SMRBC不是恒河猴B淋巴细胞的标记。此外，还与其他作者的结果进行了比较。

Abstract: The lymphocyte subpopulations in peripheral blood of Macaca mulatta,M.assamensis,M.nemestrina,M.speciosa and Tupaia belangeri and in lymphoid tissues of M.mulatta were determined with markesr,such as AET or neuraminidase treated sheep red blood cell (EAET or EVCN),zymosan-yeast-complement complex (ZYC) and stump-tailed m nkey (M.speciosa) red blood cell (SMRBC).The main results of peripheral blood lymphocytes were seen in Table 3.The percentages of EAET RFC in thymus,spleen and lymph node of M.mulatta were 95.2±2.9,28.8±10.3 and 44.8±6.2,respectively.The double marking with ZYC and SMRBC showed that SMRBC rosette formation cells of M.mulatta were not B cells.Finally,the results were compared with those from other authors.