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陈建智, 顾康福, 涂光俦. 1980: 蛇岛产蝮蛇(Agkistrodon halys pallas)蛇毒的柱层析分离及各组分的生理活性测定. 动物学研究, 1(1): 101-106.
引用本文: 陈建智, 顾康福, 涂光俦. 1980: 蛇岛产蝮蛇(Agkistrodon halys pallas)蛇毒的柱层析分离及各组分的生理活性测定. 动物学研究, 1(1): 101-106.
CHEN Jian-zhi, GU Kang-fu, TU Guang-chou. 1980. Identification of Physiological Activities in the Venom of Agkistrodon halys (From Snake Island) After Fractionation by Chromatography. Zoological Research, 1(1): 101-106.
Citation: CHEN Jian-zhi, GU Kang-fu, TU Guang-chou. 1980. Identification of Physiological Activities in the Venom of Agkistrodon halys (From Snake Island) After Fractionation by Chromatography. Zoological Research, 1(1): 101-106.

蛇岛产蝮蛇(Agkistrodon halys pallas)蛇毒的柱层析分离及各组分的生理活性测定

Identification of Physiological Activities in the Venom of Agkistrodon halys (From Snake Island) After Fractionation by Chromatography

  • 摘要: 应用二乙氨基乙基葡聚糖A-50(DEAE-Sephadex A-50)和盐浓度直线梯度洗脱的方法,对蛇岛产蝮蛇毒进行了柱层析分离,获得16个蛋白峰,并对柱层析的各组分进行了致死活性、出血活性、徐缓激肽释放活性及7种酶活力的测定。

     

    Abstract: The venom of Agkistrodon halys (From Snake Island) was fractionated on DEAE-Sephadex A-50 into sixteen different fractions.The experimental conditions are described.Some enzymatic activities (Arginine ester hydrolase,proteinase,phosphomonoesterase,phosphodiesterase,L-amino acid oxidase,5-nucleotidase and ribonuclease) as well as lethal,haemorrhagic and bradykinin releasing activities have been determined for each fraction.

     

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