蛇岛产蝮蛇(Agkistrodon halys pallas)蛇毒的柱层析分离及各组分的生理活性测定
Identification of Physiological Activities in the Venom of Agkistrodon halys (From Snake Island) After Fractionation by Chromatography
-
摘要: 应用二乙氨基乙基葡聚糖A-50(DEAE-Sephadex A-50)和盐浓度直线梯度洗脱的方法,对蛇岛产蝮蛇毒进行了柱层析分离,获得16个蛋白峰,并对柱层析的各组分进行了致死活性、出血活性、徐缓激肽释放活性及7种酶活力的测定。Abstract: The venom of Agkistrodon halys (From Snake Island) was fractionated on DEAE-Sephadex A-50 into sixteen different fractions.The experimental conditions are described.Some enzymatic activities (Arginine ester hydrolase,proteinase,phosphomonoesterase,phosphodiesterase,L-amino acid oxidase,5-nucleotidase and ribonuclease) as well as lethal,haemorrhagic and bradykinin releasing activities have been determined for each fraction.