Abstract: The venem of Agkistrodon acutus (From Fukien) was fractionated on DEAE-Sephadex A-50 into 11 different protein peaks (Fractions).The experimental conditions are described.In the crude venom,the specific activities of proteinase,arginine esterase,phosphodiesterase,as well as 5-nucleotidase are higher than that of phosphomonoesterase,phospholipase A and L-amino acid oxidase.The arginineesterase and thrombin-like activities are concentrated in fraction Ⅵ.The proteinase,fibrinolytic and anticoagulant activities are concentrated in fraction Ⅰ.Fraction Ⅸ also shows anticoagulant activity.The lethal action is located in fraction Ⅹ.The LD50 after intraperitoneal injectoin of crude venom of Agkistrodon acutus was found to be 4.4 mg/kg for mice.