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周显青, 梁洪蒙. 2003: 拥挤胁迫下小鼠肝脏脂质过氧化物含量和抗氧化物酶活性的变化. 动物学研究, 24(3): 238-240.
引用本文: 周显青, 梁洪蒙. 2003: 拥挤胁迫下小鼠肝脏脂质过氧化物含量和抗氧化物酶活性的变化. 动物学研究, 24(3): 238-240.
ZHOU Xian-qing, LIANG Hong-meng. 2003. Change in the Contents of Lipid Peroxide,and Activity of Antioxidant Enzymes in the Liver of Mice under Crowding Stress. Zoological Research, 24(3): 238-240.
Citation: ZHOU Xian-qing, LIANG Hong-meng. 2003. Change in the Contents of Lipid Peroxide,and Activity of Antioxidant Enzymes in the Liver of Mice under Crowding Stress. Zoological Research, 24(3): 238-240.

拥挤胁迫下小鼠肝脏脂质过氧化物含量和抗氧化物酶活性的变化

Change in the Contents of Lipid Peroxide,and Activity of Antioxidant Enzymes in the Liver of Mice under Crowding Stress

  • 摘要: 把实验小鼠分成2、6和10只3个密度组,饲养一周后取肝脏,采用硫代巴比妥酸法、比色法和亚硝酸盐形成法分别测定脂质过氧化物、过氧化氢酶和超氧化物歧化酶。6只组和10只组小鼠肝脏脂质过氧化物显著高于2只组;10只组过氧化氢酶活性显著低于2只组,说明拥挤降低小鼠肝脏中过氧化氢酶的活性,使脂质过氧化物含量增加,从而对机体造成过氧化损伤。

     

    Abstract: Experimental mouse were treated with three living densities,2,6,and 10 individuals per cage (26 cm×15 cm×15 cm).After a week of the treatment,livers of the mouse were collected for determining the content of lipid peroxides,and the activities of the catalase and the superoxide dismutases with thiobarbituric acid reaction,colorimetry and nitrite-form method,respectively.The content of lipid peroxides in 6 ind.-group and 10 ind.-group was significantly higher than that in 2 ind.-group.The activity of the catalase in 10 ind.-group was lower than that in 2 ind.-group,suggesting that crowding results in the decline of the catalase activity,which induces the increase of lipid peroxides,bringing peroxidatic damnification to the body.

     

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