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郭立云, 魏景宽, 杨上川, 王正波. 2012: 一种新的可用于干细胞移植的新西兰白兔青光眼视神经损伤模型. 动物学研究, (2): 225-230. DOI: 10.3724/SP.J.1141.2012.02225
引用本文: 郭立云, 魏景宽, 杨上川, 王正波. 2012: 一种新的可用于干细胞移植的新西兰白兔青光眼视神经损伤模型. 动物学研究, (2): 225-230. DOI: 10.3724/SP.J.1141.2012.02225
GUO Li-Yun, WEI Jing-Kuan, YANG Shang-Chuan, WANG Zheng-Bo. 2012: Glaucoma model for stem cell transplantation research in New Zealand white rabbits. Zoological Research, (2): 225-230. DOI: 10.3724/SP.J.1141.2012.02225
Citation: GUO Li-Yun, WEI Jing-Kuan, YANG Shang-Chuan, WANG Zheng-Bo. 2012: Glaucoma model for stem cell transplantation research in New Zealand white rabbits. Zoological Research, (2): 225-230. DOI: 10.3724/SP.J.1141.2012.02225

一种新的可用于干细胞移植的新西兰白兔青光眼视神经损伤模型

Glaucoma model for stem cell transplantation research in New Zealand white rabbits

  • 摘要: 青光眼是一种致盲且不可逆神经退行性疾病,目前还没有能够彻底解决其视野缺失的治疗方法。神经干细胞研究的兴起为该病的治疗带来了可能,且迫切需要一种适用于干细胞移植研究的青光眼造模方法。该实验对新西兰白兔球结膜下注射地塞米松给药,2.5mg/次,一周3次,持续8周。眼底照相显示造模眼眼球屈光间质保持清晰,视乳头凹陷明显扩大、血管呈屈膝状;病理切片显示造模眼视神经明显损伤;海德堡视网膜断层扫描仪(Heidelberg retina tomography,HRT)定量分析显示造模眼盘沿面积减小(1.10±0.88)mm2、杯/盘比增大0.17±0.13,视网膜神经纤维层平均厚度降低(0.44±0.31)mm等青光眼性质病理改变,且均达到极显著水平(P<0.001)。建模成功后,于角膜缘后4mm向造模眼玻璃体注入猕猴神经干细胞,植入5个月后摘取眼球切片,通过荧光显微镜观察到存活的移植细胞。该实验建立了一种简单可靠、重复性强且适用于神经干细胞移植研究的慢性青光眼造模方法。

     

    Abstract: Glaucoma is a typical irreversible blind neurodegenerative disease for which there is no effective treatment for halting visual deterioration. The recent development of neural stem cells studies sheds light on a potential resolution for this disease. As a result, an appropriate glaucoma modeling method for stem cell transplantation study is needed. In the present study, Dexamethasone was injected unilaterally into the conjunctiva of New Zealand rabbit at the dose of 2.5 mg (5 mg/mL), three times a week. After eight weeks, the eye ground photography showed that the optic nerve head of the treated eye was expanded, and the blood vessel was geniculate compared to the control eye, while the ocular media remained transparent. The hematoxylin-eosin (HE) stain of the retinal nerve fiber layer (RNFL) sections showed optic neuron death in the treated eye. The Heidelberg Retina Tomography (HRT) results showed optic disk morphological changes consistent with the pathophysiology of glaucoma in the treated eye, including a decrease in the rim area (1.10?0.88) mm2 and mean RNFL thickness (0.44?0.31) mm, and an increase in the cup/disk ratio 0.17?0.13.Then neural stem cells were injected into the vitreous body of the treated eye. After five months, surviving transplanted cells were observed. These results suggest a simple and reproducible chronic glaucoma model, which is appropriate for neural stem cell transplant research, has been successfully developed.

     

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