在大鼠玻璃体内移植猕猴神经前体细胞的方法探索
A technique of rhesus monkey neural progenitor cells intravitreal transplant to rats
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摘要: 探索一种简单、可行的异种神经前体细胞眼内移植方法。采用机械性损伤方法造成大鼠视网膜局部受损, 然后在损伤眼及对照眼玻璃体内移植绿色荧光蛋白(green fluorescence protein, GFP)标记的猕猴神经前体细胞,观察细胞能否存活。结果显示:经激光共聚焦显微镜检查发现移植细胞在损伤眼及对照眼内均可存活并整合至损伤眼视网膜。实验表明, 玻璃体内异种移植GFP 标记的猕猴神经前体细胞可以存活并整合, 是一种可行的移植方法。Abstract: To investigate a simple and effective intraocular xenotransplant technique of rhesus monkey neural progenitor cells to rats, mechanical injury was induced in the rat’s right retina. And the GFP-labeled rhesus monkey neural progenitor cells suspension was slowly injected into the vitreous space of the right injured and left control eye. Confocal image suggested that the xenografted cells survived in both the injured and control eye, meanwhile the cells integrated in the injured right retina. The results demonstrated that intravitreal xenotransplant could be adopted as a simple and reliable method.