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李明利, 田巍威, 高跃东, 郭彦, 黄京飞, 张华堂. 2012: 树鼩干扰素家族的基本构成及分子特征分析. 动物学研究, 33(1): 67-74. DOI: 10.3724/SP.J.1141.2012.01067
引用本文: 李明利, 田巍威, 高跃东, 郭彦, 黄京飞, 张华堂. 2012: 树鼩干扰素家族的基本构成及分子特征分析. 动物学研究, 33(1): 67-74. DOI: 10.3724/SP.J.1141.2012.01067
LI Ming-Li, TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, ZHANG Hua-Tang. 2012: Genome-wide prediction of interferon family members of tree shrew and their molecular characteristics analysis. Zoological Research, 33(1): 67-74. DOI: 10.3724/SP.J.1141.2012.01067
Citation: LI Ming-Li, TIAN Wei-Wei, GAO Yue-Dong, GUO Yan, HUANG Jing-Fei, ZHANG Hua-Tang. 2012: Genome-wide prediction of interferon family members of tree shrew and their molecular characteristics analysis. Zoological Research, 33(1): 67-74. DOI: 10.3724/SP.J.1141.2012.01067

树鼩干扰素家族的基本构成及分子特征分析

Genome-wide prediction of interferon family members of tree shrew and their molecular characteristics analysis

  • 摘要: 干扰素(IFN)是在“危险信号”刺激下, 由细胞分泌的具有抗病毒、抗肿瘤、抑制细胞增殖和免疫调节等多重作用的糖蛋白家族, 在机体免疫系统中具有重要地位。树鼩作为多种人类疾病研究模型的前景已受到广泛关注, 但对其IFN 家族的研究尚属空白。该研究在现有的树鼩全基因组数据基础上, 应用大片段核酸序列比对、基因预测等方法, 对树鼩IFN 家族的基本构成和分子特征进行预测和分析。结果显示, 树鼩具有I 型IFN:α (5 个亚型)、β、ω、κ、ε、δ;II 型IFN-γ;III 型IFN:IFN-λ1、λ2/3, 所编码的氨基酸序列及蛋白空间结构与其它哺乳动物具有较高的相似性, 但在半胱氨酸位置和N 糖基化个数上具有部分差异。该研究以全基因组数据对树鼩IFN家族信息进行系统挖掘和分析, 为树鼩IFN 的基因克隆以及其在感染免疫学中的作用和机理研究奠定了基础。

     

    Abstract: Interferons (IFNs) represent proteins with antiviral activities that are secreted from cells in response to a variety of stimuli. In addition to antiviral, antibacterial and anti-parasitic host-defense functions they are now also recognized as crucial regulators of cell proliferation, differentiation, survival and death as well as activators of specialized cell functions particularly in the immune system and play important roles in infectious and inflammatory diseases, autoimmunity and cancer. Tree shrews (Tupaia belangeri) were found to be susceptible to several human viruses and therefore are widely regarded as good models for analyzing mechanism of human diseases. In this report, we have forecasted the interferon family members of tree shrew from its genome mainly using the methods like Blast (whole genome shotgun sequence) and gene prediction. Our data show that tree shrew interferon system includes: type I IFN: α (five subtypes), β, ω, κ, ε, δ; type II IFN: γ; type III IFN: λ1, λ2/3. Furthermore, the predicted structures of α and λ have similar character with those of other mammals. However, there are some differences in cysteine position and N-glycosylation numbers between human and Tree shrew IFNs. These results provide fundamental basis for further molecular cloning and function analysis of tree shrew IFNs in future.

     

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