基于系统发生探讨绿带翠凤蝶和西番翠凤蝶的分类地位(英文)
Phylogenetic evaluation of the taxonomic status of Papilio maackii and P. syfanius (Lepidoptera: Papilionidae)
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摘要: 绿带翠凤蝶和西番翠凤蝶的分类问题存在一定的争议。应用分子系统学方法对这一问题进行了研究。对6个不同地区的24个绿带翠凤蝶、2个地区的16个西番翠凤蝶个体的 COI (579bp)和 COII (655bp)基因测序, 绿带翠凤蝶与西番翠凤蝶的遗传距离为0至0.6%, 共获得了15个单倍型。结果显示这些单倍型不能形成各自独立的单系群, 因此认为绿带翠凤蝶和西番翠凤蝶为近期分化的两个种Abstract: The taxonomic status of Papilio maackii and P. syfanius has long been disputed. We conducted a molecular phylogenetic study to evaluate the taxonomic status of P. maackii and P. syfanius. A total of twenty-four P. maackii individuals from six localities and sixteenP. syfanius individuals from two localities were analyzed. We sequenced the partial region of the CO-I gene (about 579bp) and partial CO-II gene sequence (about 655bp) of the two species. The Kimura-2-Parameter distances among P. maackii and P. syfanius ranged from 0 to 0.6%. Fifteen haplotypes were obtained based on the combined data set. The results strongly supported that all P. maackii individuals and all P. syfanius individuals formed a large clade, and could not be divided into separated clades. This research indicated that the two species have only very recently undergone speciation.