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杨超, 雷富民, 黄原. 2010: 地山雀线粒体基因组全序列的测定和分析. 动物学研究, 31(4): 333-344. DOI: 10.3724/SP.J.1141.2010.04333
引用本文: 杨超, 雷富民, 黄原. 2010: 地山雀线粒体基因组全序列的测定和分析. 动物学研究, 31(4): 333-344. DOI: 10.3724/SP.J.1141.2010.04333
YANG Chao, LEI Fu-Min, HUANG Yuan. 2010: Sequencing and Analysis of the Complete Mitochondrial Genome of Pseudopodoces humilis (Aves, Paridae). Zoological Research, 31(4): 333-344. DOI: 10.3724/SP.J.1141.2010.04333
Citation: YANG Chao, LEI Fu-Min, HUANG Yuan. 2010: Sequencing and Analysis of the Complete Mitochondrial Genome of Pseudopodoces humilis (Aves, Paridae). Zoological Research, 31(4): 333-344. DOI: 10.3724/SP.J.1141.2010.04333

地山雀线粒体基因组全序列的测定和分析

Sequencing and Analysis of the Complete Mitochondrial Genome of Pseudopodoces humilis (Aves, Paridae)

  • 摘要: 基于长距PCR扩增及保守引物步移法测定并注释了地山雀(Pseudopodoces humilis)的线粒体基因组全序列。结果表明,地山雀线粒体基因组全长1.6 809 万 bp,A+T含量为52.9%,37个基因排列顺序与红原鸡一致。蛋白质基因的起始密码子中,除COI基因为GTG外,其余均为ATG。ND1和ND5基因终止密码子为AGA;COII基因为AGG;COIII和ND4基因为不完全终止密码子T;其余基因均为典型的TAA或TAG。预测了22个tRNA基因的二级结构,发现tRNASer (AGN)缺少DHU臂,tRNAPhe的TψC臂存在一单核苷酸插入。预测的地山雀12S和16S rRNA二级结构分别包括3个结构域47个茎环和6个结构域60个茎环。 控制区位于tRNAGlu和 tRNAPhe之间,长度1 240 bp。控制区结构为高变I区、中央保守II区和保守序列III区3个结构域。

     

    Abstract: The complete sequence of Pseudopodoces humilis mitochondrial genome was determined by using long PCR and conserved primers walking approaches. The results showed that the entire mitochondrial genome of Pseudopodoces humilis was 16 809 bp in length with 52.9% A+T content, and the 37 genes had the same gene order with that of Gallus gallus. All protein coding genes of the Pseudopodoces humilis mitochondrial genome started with ATG codon, except for COIwith GTG. For terminate codon usage, most of genes terminate with codons TAA or TAG, ND1 and ND5 were AGA, COII was AGG, and the COIII and ND4 genes had an incomplete termination codon (T). The secondary structures of 22 tRNA were predicted, all tRNA can form typical secondary structure, except tRNASer ( AGN ) with an absence of DHU arm, and tRNAPhe with an extra nucleotide inserted in the TψC arm. The predicted secondary structures of 12S rRNA and 16S rRNA exhibit 47 helics in 3 structural domains and 60 helics in 6 domains, respectively. The control region of Pseudopodoces humilis located between tRNAGlu and tRNAPhe with 1 240 bp in length, and has structure domains found in that of the other birds.

     

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