Abstract: Interleukin-1β(IL-1β) is an important porinflammatory cytokine that exert wide biological functions in the central nervous system (CNS). Voltage-gated Ca²⁺ channels in plasma membrane are essential components of Ca²⁺ signal whose change on pathological conditions is closely related to processes of diseases. Although both IL-1βand Ca²⁺ channels have key roles in injury and disease of the brain, so far their relations have been reported in few studies. The effects of IL-1βon Ca²⁺ channels in cultured cortical neurons of rats were investigated using patch-clamp recording in present research. Our results showed that both 10 and 50 ng/mL of IL-1βtreatment inhibit the Ca²⁺ currents in time and dose dependent manner, without the change of activation properties of voltage-gated Ca²⁺ channels (VGCC)s.