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陈欣欣, 余果宇, 张彦, 张云, 申吉泓, 李文辉. 2009: 抗菌肽OH-CATH对大肠杆菌Escherichia coli的作用(英文). 动物学研究, 30(2): 171-177. DOI: 10.3724/SP.J.1141.2009.02171
引用本文: 陈欣欣, 余果宇, 张彦, 张云, 申吉泓, 李文辉. 2009: 抗菌肽OH-CATH对大肠杆菌Escherichia coli的作用(英文). 动物学研究, 30(2): 171-177. DOI: 10.3724/SP.J.1141.2009.02171
Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. 2009: Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli. Zoological Research, 30(2): 171-177. DOI: 10.3724/SP.J.1141.2009.02171
Citation: Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. 2009: Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli. Zoological Research, 30(2): 171-177. DOI: 10.3724/SP.J.1141.2009.02171

抗菌肽OH-CATH对大肠杆菌Escherichia coli的作用(英文)

Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli

  • 摘要: OH-CATH是眼镜王蛇中新发现的cathelicidin家族抗菌肽。它在1% NaCl存在的条件下对多种细菌都有较强的抗菌活性,同时,在高浓度下对人红细胞无溶血活性。OH-CATH是开发新型抗菌药物的优良模板。阐明OH-CATH的作用机理及其对微生物的选择性,对研发以OH-CATH为先导结构的药物研发有十分重要的意义。本文利用扫描电镜以及透射电镜对OH-CATH与革兰氏阴性菌-大肠杆菌ATCC 25922相互作用的效应研究。结果揭示:OH-CATH对大肠杆菌的作用涉及到3个步骤。首先,OH-CATH借助其带正电的氨基酸残基附着到细菌带负电荷的细胞壁;然后,附着的OH-CATH在达到一定浓度后发生聚集,以孔道形成的方式破坏细菌的膜结构;最终,由于细菌膜的损坏,膜的渗透性被破坏,胞内内含物释放造成细菌死亡。

     

    Abstract: OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCl and no haemolytic activity toward human red blood cells even at a high concentration. OH-CATH might serve as model molecules for the development of antimicrobial drugs. Understanding the action mechanism of OH-CATH and the reason for its selectivity against microbes is very important for this purpose. The bactericidal effect of the king cobra antimicrobial peptide OH-CATH on Gram-negative Escherichia coli (ATCC 25922) is observed by scanning electron microscopy (SEM) and transmitted electron microscopy (TEM). The SEM and TEM results suggested that the bactericidal mechanism of OH-CATH against Escherichia coli happened in three steps. Firstly, OH-CATH attached to the negatively charged bacterial wall by positively charged amino acid residues. In the second step, the accumulated OH-CATH aggregated and damaged the bacteria membrane in a pore-forming manner. In the last step, with the damage of cell permeability, the contents of the cells were released and eventually cells died.

     

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