• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
黄 琳, 张锐, 宿 兵, . 2008: PLOD3基因3′调控区的人类特异突变改变miR-124a对PLOD3的调控. 动物学研究, 29(4): 363-367. DOI: 10.3724/SP.J.1141.2008.04363
引用本文: 黄 琳, 张锐, 宿 兵, . 2008: PLOD3基因3′调控区的人类特异突变改变miR-124a对PLOD3的调控. 动物学研究, 29(4): 363-367. DOI: 10.3724/SP.J.1141.2008.04363
HUANG Lin , , ZHANG Rui , SU Bing. 2008: A Human-specific Mutation Leads to Reduced Interaction Between miR-124a and One of Its Target Genes, PLOD3. Zoological Research, 29(4): 363-367. DOI: 10.3724/SP.J.1141.2008.04363
Citation: HUANG Lin , , ZHANG Rui , SU Bing. 2008: A Human-specific Mutation Leads to Reduced Interaction Between miR-124a and One of Its Target Genes, PLOD3. Zoological Research, 29(4): 363-367. DOI: 10.3724/SP.J.1141.2008.04363

PLOD3基因3′调控区的人类特异突变改变miR-124a对PLOD3的调控

A Human-specific Mutation Leads to Reduced Interaction Between miR-124a and One of Its Target Genes, PLOD3

  • 摘要: microRNAs(miRNAs)是一类在转录后水平调控基因表达的不编码蛋白质的小RNA(长度20—24个碱基)。其中,miR-124a是一个在哺乳动物中枢神经系统高度表达的miRNA,在神经前体细胞向神经元分化的过程中起着举足轻重的作用。由于miRNAs特异性地识别靶基因的3′端调控区(3′UTR)的靶序列,因此,在人类起源过程中基因3′UTR的单核苷酸序列变异有可能导致miRNA调控的改变。通过靶基因预测和3′UTR区在哺乳动物代表物种间的同源序列比较,我们发现miR-124a的靶基因中有一个基因(PLOD3)3′UTR的靶位点中存在人类特异突变位点。利用体外报告基因系统,发现PLOD3基因3′UTR靶位点中所含的一个人类特异的突变导致miR-124a对PLOD3的调控效率降低。研究表明,miRNAs靶基因3′UTR的序列变异具有功能效应,它有可能是人类中枢神经系统在起源和演化中发挥关键作用的重要遗传机制之一。

     

    Abstract: MicroRNAs (miRNAs)are a class of noncoding RNAs (20-24 nt) that can post-transcriptionally regulate the expression of protein coding genes in metazoans. Among them, miR-124a, which is preferentially expressed in the brain, controls the neuronal differentiation in mammals. As miRNAs recognize sequences in the 3′ untranslated region (UTR) of the target genes, during human origin, mutations located in the 3′UTRs of the target genes may lead to changes in miRNA regulation. Through target gene prediction and comparative sequence analysis in representative mammalian species, we identified a target gene (PLOD3) of miR-124a, which has a human-specific mutation in the 3′UTR target sequence. Using the in vitro reporter gene system, we discovered that the human specific mutation in the target site of PLOD3 leads to reduced interaction between miR-124a and PLOD3. This result implies that sequence changes located in the 3′UTR segment of the target genes may have functional consequence and eventually contribute to the origin and evolution of human cognition.

     

/

返回文章
返回