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Lorenzo Alibardi. 2019: 蜥蜴(Podarcis muralis)再生尾ephrin受体刺激分化和肌肉分节. 动物学研究, 40(5): 416-426. DOI: 10.24272/j.issn.2095-8137.2019.046
引用本文: Lorenzo Alibardi. 2019: 蜥蜴(Podarcis muralis)再生尾ephrin受体刺激分化和肌肉分节. 动物学研究, 40(5): 416-426. DOI: 10.24272/j.issn.2095-8137.2019.046
Lorenzo Alibardi. 2019: Immunodetection of ephrin receptors in the regenerating tail of the lizard Podarcis muralis suggests stimulation of differentiation and muscle segmentation. Zoological Research, 40(5): 416-426. DOI: 10.24272/j.issn.2095-8137.2019.046
Citation: Lorenzo Alibardi. 2019: Immunodetection of ephrin receptors in the regenerating tail of the lizard Podarcis muralis suggests stimulation of differentiation and muscle segmentation. Zoological Research, 40(5): 416-426. DOI: 10.24272/j.issn.2095-8137.2019.046

蜥蜴(Podarcis muralis)再生尾ephrin受体刺激分化和肌肉分节

Immunodetection of ephrin receptors in the regenerating tail of the lizard Podarcis muralis suggests stimulation of differentiation and muscle segmentation

  • 摘要: 酪氨酸蛋白激酶受体(ephrin受体)是发育过程中最常见的酪氨酸激酶的效应器。有报道发现蜥蜴(Podarcis muralis)尾部再生时,ephrin受体基因水平上调。因此,在本研究中,我们检测蜥蜴再生尾中ephrin受体的免疫定位。ephrin受体的弱免疫标记带在15–17kDa,强免疫标记带同时在60–65kDa处也可检测到。结果发现:在刺激蜥蜴尾部再生的两个关键组织——创伤面的表皮和室管膜的基底层,可见标记受体蛋白的细胞和细胞核。再生尾分节肌肉、稀疏血管和再生软骨的软骨膜中存在强的细胞核和细胞质标记,提示新生肌肉组织中分布的大量ephrin受体可能与分节相关。该研究表明,分布于分化的表皮、室管膜、肌肉和软骨细胞中大量的ephrin受体,可能通过激活再生尾近端的细胞分化程序,从而调节细胞增殖。而顶端芽基中的ephrin受体免疫标记较少,取而代之存在信号蛋白,可刺激细胞增殖,有助于维持芽基的持续生长。

     

    Abstract: Ephrin receptors are the most common tyrosine kinase effectors operating during development. Ephrin receptor genes are reported to be up-regulated in the regenerating tail of the Podarcis muralis lizard. Thus, in the current study, we investigated immunolocalization of ephrin receptors in the Podarcis muralis tail during regeneration. Weak immunolabelled bands for ephrin receptors were detected at 15–17 kDa, with a stronger band also detected at 60–65 kDa. Labelled cells and nuclei were seen in the basal layer of the apical wound epidermis and ependyma, two key tissues stimulating tail regeneration. Strong nuclear and cytoplasmic labelling were present in the segmental muscles of the regenerating tail, sparse blood vessels, and perichondrium of regenerating cartilage. The immunolocalization of ephrin receptors in muscle that gives rise to large portions of new tail tissue was correlated with their segmentation. This study suggests that the high localization of ephrin receptors in differentiating epidermis, ependyma, muscle, and cartilaginous cells is connected to the regulation of cell proliferation through the activation of programs for cell differentiation in the proximal regions of the regenerating tail. The lower immunolabelling of ephrin receptors in the apical blastema, where signaling proteins stimulating cell proliferation are instead present, helps maintain the continuous growth of this region.

     

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